7lca

X-ray diffraction
2Å resolution

Zoogloea ramigera biosynthetic thiolase Y218E/delH221 mutant

Released:
Model geometry
Fit model/data
Source organism: Zoogloea ramigera
Primary publication:
Engineering potassium activation into biosynthetic thiolase.
Biochem J 478 3047-3062 (2021)
PMID: 34338286

Function and Biology Details

Reaction catalysed:
(1a) acetyl-CoA + [acetyl-CoA C-acetyltransferase]-L-cysteine = [acetyl-CoA C-acetyltransferase]-S-acetyl-L-cysteine + CoA

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
PDBe Complex ID:
PDB-CPX-139369 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Acetyl-CoA acetyltransferase Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 398 amino acids
Theoretical weight: 41.37 KDa
Source organism: Zoogloea ramigera
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P07097 (Residues: 2-11, 12-392; Coverage: 100%)
Gene names: phaA, phbA
Sequence domains:

Ligands and Environments


Cofactor: Ligand COA 4 x COA
2 bound ligands:
1 modified residue:

Experiments and Validation Details

wwPDB Validation report is not available for this entry.
X-ray source: AUSTRALIAN SYNCHROTRON BEAMLINE MX1
Spacegroup: P21
Unit cell:
a: 84.45Å b: 79.34Å c: 149.14Å
α: 90° β: 92.86° γ: 90°
R-values:
R R work R free
0.326 0.261 0.283
Expression system: Escherichia coli BL21(DE3)