› 7ljs

X-ray diffraction
2Å resolution

Porcine Dihydropyrimidine dehydrogenase (DPD) complexed with 5-Ethynyluracil (5EU) - Open Form

Released:
DOI: 10.2210/pdb7ljs/pdb
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Source organism: Sus scrofa
Primary publication:
The Interaction of Porcine Dihydropyrimidine Dehydrogenase with the Chemotherapy Sensitizer: 5-Ethynyluracil.
Forouzesh DC, Beaupre BA, Butrin A, Wawrzak Z, Liu D, Moran GR
Biochemistry 60 1120-1132 (2021)
PMID: 33755421

Function and Biology Details

Reaction catalysed: 
5,6-dihydrouracil + NADP(+) = uracil + NADPH
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-173320 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Dihydropyrimidine dehydrogenase [NADP(+)] Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 1025 amino acids
Theoretical weight: 111.6 KDa
Source organism: Sus scrofa
Expression system: Escherichia coli
UniProt:
  • Canonical: Q28943 (Residues: 1-1025; Coverage: 100%)
Gene name: DPYD
Sequence domains:

Ligands and Environments


Cofactor: Ligand FAD 4 x FAD

Cofactor: Ligand FMN 4 x FMN
2 bound ligands:
Ligand SF4 16 x SF4
Ligand Y3G 4 x Y3G
No modified residues

Experiments and Validation Details

wwPDB Validation report is not available for this entry.
X-ray source: APS BEAMLINE 21-ID-F
Spacegroup: P21
Unit cell:
a: 82.031Å b: 160.046Å c: 164.074Å
α: 90° β: 95.95° γ: 90°
R-values:
R R work R free
0.175 0.172 0.229
Expression system: Escherichia coli

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