› 7ll2

Electron Microscopy
3.73Å resolution

Cryo-EM structure of BG505 DS-SOSIP in complex with Glycan276-Dependent Broadly Neutralizing Antibody VRC33.01 Fab

Released:
DOI: 10.2210/pdb7ll2/pdb
PDB entry 7ll2 coloured by chain, front view.
PDB entry 7ll2 coloured by chain, side view.
PDB entry 7ll2 coloured by chain, top view.
Source organisms:
Primary publication:
Structural basis of glycan276-dependent recognition by HIV-1 broadly neutralizing antibodies.
Cottrell CA, Manne K, Kong R, Wang S, Zhou T, Chuang GY, Edwards RJ, Henderson R, Janowska K, Kopp M, Lin BC, Louder MK, Olia AS, Rawi R, Shen CH, Taft JD, Torres JL, Wu NR, Zhang B, Doria-Rose NA, Cohen MS, Haynes BF, Shapiro L, Ward AB, Acharya P, Mascola JR, Kwong PD
OpenAccess logo Cell Rep 37 109922 (2021)
PMID: 34731616
Related structures: EMD-23412

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero dodecamer (preferred)
PDBe Complex ID:
PDB-CPX-212200 (preferred)
Entry contents:
4 distinct polypeptide molecules
Macromolecules (8 distinct):
Envelope glycoprotein gp160 Chains: A, C, E
Molecule details ›
Chains: A, C, E
Length: 473 amino acids
Theoretical weight: 52.99 KDa
Source organism: Human immunodeficiency virus 1
Expression system: Homo sapiens
UniProt:
  • Canonical: Q2N0S6 (Residues: 30-502; Coverage: 55%)
Gene name: env
Sequence domains: Envelope glycoprotein GP120
Transmembrane protein gp41 Chains: B, D, F
Molecule details ›
Chains: B, D, F
Length: 153 amino acids
Theoretical weight: 17.16 KDa
Source organism: Human immunodeficiency virus 1
Expression system: Homo sapiens
UniProt:
  • Canonical: Q2N0S7 (Residues: 509-661; Coverage: 18%)
Gene name: env
Sequence domains: Retroviral envelope protein
VRC33.01 Fab Heavy chain Chains: H, I, K
VRC33.01 Fab Light chain Chains: J, L, M

Ligands and Environments

Carbohydrate polymer : NEW Components: NAG, BMA
Carbohydrate polymer
Carbohydrate polymer : NEW Components: NAG
Carbohydrate polymer
Carbohydrate polymer : NEW Components: NAG, BMA, MAN
Carbohydrate polymer
Carbohydrate polymer : NEW Components: NAG, BMA, MAN
Carbohydrate polymer
1 bound ligand:
Ligand NAG 12 x NAG
No modified residues

Experiments and Validation Details

wwPDB Validation report is not available for this EM entry.
Resolution: 3.73Å
Relevant EMDB volumes: EMD-23412
Expression system: Homo sapiens

Quick links

Citations

1 review citation

Cryo-electron microscopy in the study of virus entry and infection.
Dutta et al. (2024)
4 other articles

1 mention without citation

Structural basis of glycan276-dependent recognition by HIV-1 broadly neutralizing antibodies.
Cottrell et al. (2021)