› 7lt9

X-ray diffraction
3.05Å resolution

Crystal structure of Ras suppressor-1 in complex with PINCH-1 LIM4-5 domains

Released:
DOI: 10.2210/pdb7lt9/pdb
PDB entry 7lt9 coloured by chain, front view.
PDB entry 7lt9 coloured by chain, side view.
PDB entry 7lt9 coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Molecular basis for Ras suppressor-1 binding to PINCH-1 in focal adhesion assembly.
Fukuda K, Lu F, Qin J
J Biol Chem 296 100685
PMID: 33891945

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-155701 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Ras suppressor protein 1 Chain: A
Molecule details ›
Chain: A
Length: 280 amino acids
Theoretical weight: 31.86 KDa
Source organism: Homo sapiens
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: Q15404 (Residues: 1-277; Coverage: 100%)
Gene names: RSP1, RSU1
Sequence domains: Leucine rich repeat
LIM and senescent cell antigen-like-containing domain protein 1 Chain: B
Molecule details ›
Chain: B
Length: 141 amino acids
Theoretical weight: 16.19 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P48059 (Residues: 189-325; Coverage: 42%)
Gene names: LIMS1, PINCH, PINCH1
Sequence domains: LIM domain

Ligands and Environments

1 bound ligand:
Ligand ZN 4 x ZN
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 19-BM
Spacegroup: I222
Unit cell:
a: 51.042Å b: 144.384Å c: 185.691Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.201 0.2 0.234
Expression systems:
  • Spodoptera frugiperda
  • Escherichia coli

Quick links

Citations

1 citations in other articles

Comprehensive analysis of cell-extracellular matrix protein Ras suppressor-1 in function and prognosis of gastrointestinal cancers.
Xu et al. (2023)

1 mention without citation

Molecular basis for Ras suppressor-1 binding to PINCH-1 in focal adhesion assembly.
Fukuda et al. (2021)