› 7lz4

X-ray diffraction
4.16Å resolution

Crystal structure of A211D mutant of Protein Kinase A RIa subunit, a Carney Complex mutation

Released:
DOI: 10.2210/pdb7lz4/pdb
Model geometry
Fit model/data
PDB entry 7lz4 coloured by chain, front view.
PDB entry 7lz4 coloured by chain, side view.
PDB entry 7lz4 coloured by chain, top view.
Source organism: Bos taurus
Primary publication:
Noncanonical protein kinase A activation by oligomerization of regulatory subunits as revealed by inherited Carney complex mutations.
Jafari N, Del Rio J, Akimoto M, Byun JA, Boulton S, Moleschi K, Alsayyed Y, Swanson P, Huang J, Martinez Pomier K, Lee C, Wu J, Taylor SS, Melacini G
Proc Natl Acad Sci U S A 118 (2021)
PMID: 34006641

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-132578 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
cAMP-dependent protein kinase type I-alpha regulatory subunit, N-terminally processed Chains: A, B, C, D, E, F, G, H
Molecule details ›
Chains: A, B, C, D, E, F, G, H
Length: 269 amino acids
Theoretical weight: 30.34 KDa
Source organism: Bos taurus
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P00514 (Residues: 109-377; Coverage: 71%)
Gene name: PRKAR1A
Sequence domains: Cyclic nucleotide-binding domain

Ligands and Environments

1 bound ligand:
Ligand CMP 16 x CMP
No modified residues

Experiments and Validation Details

wwPDB Validation report is not available for this entry.
X-ray source: ALS BEAMLINE 8.2.2
Spacegroup: R3
Unit cell:
a: 176.786Å b: 176.786Å c: 345.503Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.224 0.221 0.27
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

1 review citation

From structure to the dynamic regulation of a molecular switch: A journey over 3 decades.
Taylor et al. (2021)