› 7m2x

Electron Microscopy
3.6Å resolution

Open conformation of the Yeast wild-type gamma-TuRC

Released:
DOI: 10.2210/pdb7m2x/pdb
PDB entry 7m2x coloured by chain, front view.
PDB entry 7m2x coloured by chain, side view.
PDB entry 7m2x coloured by chain, top view.
Source organism: Saccharomyces cerevisiae S288C
Primary publication:
CM1-driven assembly and activation of yeast γ-tubulin small complex underlies microtubule nucleation.
Brilot AF, Lyon AS, Zelter A, Viswanath S, Maxwell A, MacCoss MJ, Muller EG, Sali A, Davis TN, Agard DA
Elife 10 (2021)
PMID: 33949948
Related structures: EMD-23636

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero pentamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-152291 (preferred)
Entry contents:
4 distinct polypeptide molecules
Macromolecules (4 distinct):
Tubulin gamma chain Chains: A, B
Molecule details ›
Chains: A, B
Length: 473 amino acids
Theoretical weight: 52.67 KDa
Source organism: Saccharomyces cerevisiae S288C
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: P53378 (Residues: 1-473; Coverage: 100%)
Gene names: L8167.21, TUB4, YLR212C
Sequence domains:
Spindle pole body component SPC97 Chain: C
Molecule details ›
Chain: C
Length: 823 amino acids
Theoretical weight: 96.94 KDa
Source organism: Saccharomyces cerevisiae S288C
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: P38863 (Residues: 1-823; Coverage: 100%)
Gene names: SPC97, YHR172W
Sequence domains:
Spindle pole body component SPC98 Chain: D
Molecule details ›
Chain: D
Length: 846 amino acids
Theoretical weight: 98.34 KDa
Source organism: Saccharomyces cerevisiae S288C
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: P53540 (Residues: 1-846; Coverage: 100%)
Gene names: N1222, N1879, SPC98, YNL126W
Sequence domains:
Spindle pole body component 110 Chain: U
Molecule details ›
Chain: U
Length: 220 amino acids
Theoretical weight: 25.45 KDa
Source organism: Saccharomyces cerevisiae S288C
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: P32380 (Residues: 1-220; Coverage: 23%)
Gene names: D9476.3, NUF1, SPC110, XCM1, YDR356W

Ligands and Environments

1 bound ligand:
Ligand GDP 2 x GDP
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 3.6Å
Relevant EMDB volumes: EMD-23636
Expression system: Spodoptera frugiperda

Quick links

Citations

4 review citations

Molecular insight into how γ-TuRC makes microtubules.
Thawani et al. (2021)
3 more

1 mention without citation

CM1-driven assembly and activation of yeast γ-tubulin small complex underlies microtubule nucleation.
Brilot et al. (2021)