7m3t

X-ray diffraction
3.2Å resolution

Crystallographic structure of a cubic crystal of STMV (80.7 degree rotation about 111) grown from chloride

Released:
Primary publication:
Structures of additional crystal forms of Satellite tobacco mosaic virus grown from a variety of salts.
Acta Crystallogr F Struct Biol Commun 77 473-483 (2021)
PMID: 34866603

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:

Structure analysis Details

Assembly composition:
hetero 117-mer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-148089 (preferred)
Entry contents:
3 distinct polypeptide molecules
7 distinct RNA molecules
Macromolecules (10 distinct):
Coat protein Chains: A, B, C, D, E, F, GG, H, HH, I, II, J, JJ, K, KK, L, M, O
Molecule details ›
Chains: A, B, C, D, E, F, GG, H, HH, I, II, J, JJ, K, KK, L, M, O
Length: 159 amino acids
Theoretical weight: 17.53 KDa
Source organism: Satellite tobacco mosaic virus
UniProt:
  • Canonical: P17574 (Residues: 1-159; Coverage: 100%)
Coat protein Chain: G
Molecule details ›
Chain: G
Length: 159 amino acids
Theoretical weight: 17.52 KDa
Source organism: Satellite tobacco mosaic virus
UniProt:
  • Canonical: P17574 (Residues: 1-159; Coverage: 100%)
Coat protein Chain: N
Molecule details ›
Chain: N
Length: 159 amino acids
Theoretical weight: 17.56 KDa
Source organism: Satellite tobacco mosaic virus
UniProt:
  • Canonical: P17574 (Residues: 1-159; Coverage: 100%)
RNA (5'-R(P*AP*AP*AP*AP*AP*AP*AP*AP*AP*A)-3') Chains: P, S, T, TT, UU, V, X, Y, a
Molecule details ›
Chains: P, S, T, TT, UU, V, X, Y, a
Length: 10 nucleotides
Theoretical weight: 3.25 KDa
RNA (5'-R(P*UP*UP*UP*UP*UP*UP*UP*UP*UP*UP*UP*U)-3') Chains: WW, e
Molecule details ›
Chains: WW, e
Length: 12 nucleotides
Theoretical weight: 3.63 KDa
RNA (5'-R(P*UP*AP*UP*UP*UP*UP*UP*UP*UP*UP*UP*U)-3') Chain: h
Molecule details ›
Chain: h
Length: 12 nucleotides
Theoretical weight: 3.65 KDa
RNA (5'-R(P*UP*UP*UP*UP*UP*UP*UP*UP*UP*UP*U)-3') Chains: i, ll
Molecule details ›
Chains: i, ll
Length: 11 nucleotides
Theoretical weight: 3.32 KDa
RNA (5'-R(P*UP*UP*UP*UP*UP*UP*UP*UP*UP*U)-3') Chains: kk, m
Molecule details ›
Chains: kk, m
Length: 10 nucleotides
Theoretical weight: 3.02 KDa
RNA (5'-R(P*UP*UP*UP*UP*UP*UP*UP*U)-3') Chains: n, qq
Molecule details ›
Chains: n, qq
Length: 8 nucleotides
Theoretical weight: 2.4 KDa
RNA (5'-R(P*AP*AP*AP*AP*AP*AP*AP*AP*AP*AP*AP*A)-3') Chain: bb
Molecule details ›
Chain: bb
Length: 12 nucleotides
Theoretical weight: 3.91 KDa

Ligands and Environments

3 bound ligands:
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ALS BEAMLINE 8.3.1
Spacegroup: P23
Unit cell:
a: 234.05Å b: 234.05Å c: 234.05Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.218 0.215 0.254