› 7m4p

Electron Microscopy
2.86Å resolution

Multidrug Efflux pump AdeJ with Eravacycline bound

Released:
DOI: 10.2210/pdb7m4p/pdb
PDB entry 7m4p coloured by chain, front view.
PDB entry 7m4p coloured by chain, side view.
PDB entry 7m4p coloured by chain, top view.
Source organism: Acinetobacter baumannii
Primary publication:
Cryo-EM Determination of Eravacycline-Bound Structures of the Ribosome and the Multidrug Efflux Pump AdeJ of Acinetobacter baumannii.
Zhang Z, Morgan CE, Bonomo RA, Yu EW
mBio 12 e0103121 (2021)
PMID: 34044590
Related structures: EMD-23663

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-173395 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Efflux pump membrane transporter Chains: A, B, C
Molecule details ›
Chains: A, B, C
Length: 1058 amino acids
Theoretical weight: 114.64 KDa
Source organism: Acinetobacter baumannii
Expression system: Escherichia coli K-12
UniProt:
  • Canonical: Q2FD94 (Residues: 1-1058; Coverage: 100%)
Gene names: 16_18, A7M90_18260, ABR2091_2941, ABUW_0843, B9W25_10035, B9X95_17010, CAS83_01890, CBE85_16500, CV954_003755, DOL94_01575, F2P40_15340, F4T85_03325, FJU42_11415, G3N53_18475, GNY86_00640, GSE42_16275, IAG11_12040, ITE13_15860, JHZ39_002181, MKP18_003079, SAMEA104305318_02843, SAMEA4394745_03226, adeJ, mexB_2
Sequence domains: AcrB/AcrD/AcrF family

Ligands and Environments

2 bound ligands:
Ligand 3PE 8 x 3PE
Ligand YQM 1 x YQM
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 2.86Å
Relevant EMDB volumes: EMD-23663
Expression system: Escherichia coli K-12

Quick links

Citations

2 review citations

Drug‑resistant Acinetobacter baumannii: From molecular mechanisms to potential therapeutics (Review).
Wu et al. (2023)
1 more

4 mentions without citation

Bacterial effluxome as a barrier against antimicrobial agents: structural biology aspects and drug targeting.
Brindangnanam et al. (2022)
3 more