› 7m50

X-ray diffraction
2.31Å resolution

Crystallographic structure of a cubic crystal form of STMV grown from ammonium sulfate

Released:
DOI: 10.2210/pdb7m50/pdb
PDB entry 7m50 coloured by chain, front view.
PDB entry 7m50 coloured by chain, side view.
PDB entry 7m50 coloured by chain, top view.
Source organism: Satellite tobacco mosaic virus
Primary publication:
Structures of additional crystal forms of Satellite tobacco mosaic virus grown from a variety of salts.
McPherson A
Acta Crystallogr F Struct Biol Commun 77 473-483 (2021)
PMID: 34866603

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
Sequence domain:

Structure analysis Details

Assembly composition:
hetero 117-mer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-148099 (preferred)
Entry contents:
1 distinct polypeptide molecule
8 distinct RNA molecules
Macromolecules (9 distinct):
Coat protein Chains: A, B, C, D, E, F, G, GG, H, HH, I, II, J, JJ, K, KK, L, M, N, O
Molecule details ›
Chains: A, B, C, D, E, F, G, GG, H, HH, I, II, J, JJ, K, KK, L, M, N, O
Length: 159 amino acids
Theoretical weight: 17.53 KDa
Source organism: Satellite tobacco mosaic virus
UniProt:
  • Canonical: P17574 (Residues: 1-159; Coverage: 100%)
RNA (5'-R(P*AP*AP*AP*AP*AP*AP*AP*A)-3') Chains: P, TT, V
Molecule details ›
Chains: P, TT, V
Length: 8 nucleotides
Theoretical weight: 2.59 KDa
Source organism: Satellite tobacco mosaic virus
RNA (5'-R(P*AP*AP*AP*AP*AP*A)-3') Chains: S, T
Molecule details ›
Chains: S, T
Length: 6 nucleotides
Theoretical weight: 1.93 KDa
Source organism: Satellite tobacco mosaic virus
RNA (5'-R(P*UP*UP*UP*UP*UP*UP*UP*UP*UP*U)-3') Chains: UU, X, bb
Molecule details ›
Chains: UU, X, bb
Length: 10 nucleotides
Theoretical weight: 3.25 KDa
Source organism: Satellite tobacco mosaic virus
RNA (5'-R(P*UP*UP*UP*UP*UP*UP*U)-3') Chains: Y, a
Molecule details ›
Chains: Y, a
Length: 7 nucleotides
Theoretical weight: 2.26 KDa
Source organism: Satellite tobacco mosaic virus
RNA (5'-R(P*UP*UP*UP*UP*UP*UP*UP*UP*UP*UP*UP*U))-3') Chains: WW, e
Molecule details ›
Chains: WW, e
Length: 12 nucleotides
Theoretical weight: 3.63 KDa
Source organism: Satellite tobacco mosaic virus
RNA (5'-R(P*UP*UP*UP*UP*UP*UP*UP*UP*UP*U)-3') Chain: h
Molecule details ›
Chain: h
Length: 10 nucleotides
Theoretical weight: 3.02 KDa
Source organism: Satellite tobacco mosaic virus
RNA (5'-R(P*UP*UP*UP*UP*UP*UP*UP*UP*U)-3') Chains: i, ll, qq
Molecule details ›
Chains: i, ll, qq
Length: 9 nucleotides
Theoretical weight: 2.71 KDa
Source organism: Satellite tobacco mosaic virus
RNA (5'-R(P*UP*UP*UP*UP*UP*UP*UP*U)-3') Chains: kk, m, n
Molecule details ›
Chains: kk, m, n
Length: 8 nucleotides
Theoretical weight: 2.4 KDa
Source organism: Satellite tobacco mosaic virus

Ligands and Environments

4 bound ligands:
Ligand PO4 6 x PO4
Ligand MG 5 x MG
Ligand CL 1 x CL
Ligand SO4 6 x SO4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ALS BEAMLINE 8.3.1
Spacegroup: P23
Unit cell:
a: 234.05Å b: 234.05Å c: 234.05Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.248 0.246 0.271

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Citations

1 mention without citation

Structures of additional crystal forms of Satellite tobacco mosaic virus grown from a variety of salts.
McPherson A. (2021)