› 7m55

X-ray diffraction
1.4Å resolution

B6 Fab fragment bound to the MERS-CoV spike stem helix peptide

Released:
DOI: 10.2210/pdb7m55/pdb
PDB entry 7m55 coloured by chain, front view.
PDB entry 7m55 coloured by chain, side view.
PDB entry 7m55 coloured by chain, top view.
Source organisms:
Primary publication:
Structural basis for broad coronavirus neutralization.
Sauer MM, Tortorici MA, Park YJ, Walls AC, Homad L, Acton OJ, Bowen JE, Wang C, Xiong X, de van der Schueren W, Quispe J, Hoffstrom BG, Bosch BJ, McGuire AT, Veesler D
Nat Struct Mol Biol 28 478-486 (2021)
PMID: 33981021

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-207959 (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
Spike protein S2' Chain: A
Molecule details ›
Chain: A
Length: 15 amino acids
Theoretical weight: 1.83 KDa
Source organism: Middle East respiratory syndrome-related coronavirus
Expression system: Not provided
UniProt:
  • Canonical: K9N5Q8 (Residues: 1230-1244; Coverage: 1%)
Gene names: 3, S
B6 antigen binding fragment (Fab) heavy chain Chain: H
Molecule details ›
Chain: H
Length: 220 amino acids
Theoretical weight: 23.35 KDa
Source organism: Mus musculus
Expression system: Homo sapiens
B6 antigen binding fragment (Fab) light chain Chain: L
Molecule details ›
Chain: L
Length: 219 amino acids
Theoretical weight: 24 KDa
Source organism: Mus musculus
Expression system: Homo sapiens

Ligands and Environments

1 bound ligand:
Ligand GOL 3 x GOL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ALS BEAMLINE 5.0.1
Spacegroup: C2
Unit cell:
a: 93.589Å b: 60.444Å c: 79.71Å
α: 90° β: 93.75° γ: 90°
R-values:
R R work R free
0.174 0.173 0.2
Expression systems:
  • Not provided
  • Homo sapiens

Quick links

Citations

16 review citations

Antiviral Drug Discovery for the Treatment of COVID-19 Infections.
Ng et al. (2022)
15 more

2 mentions without citation

Antibody-mediated immunity to SARS-CoV-2 spike.
Errico et al. (2022)
1 more