› 7mcb

X-ray diffraction
2.14Å resolution

Crystal structure of Staphylococcus aureus Cystathionine gamma lyase Holoenzyme

Released:
DOI: 10.2210/pdb7mcb/pdb
Model geometry
Fit model/data
PDB entry 7mcb coloured by chain, front view.
PDB entry 7mcb coloured by chain, side view.
PDB entry 7mcb coloured by chain, top view.
Source organism: Staphylococcus aureus
Primary publication:
Inhibitors of bacterial H2S biogenesis targeting antibiotic resistance and tolerance.
Shatalin K, Nuthanakanti A, Kaushik A, Shishov D, Peselis A, Shamovsky I, Pani B, Lechpammer M, Vasilyev N, Shatalina E, Rebatchouk D, Mironov A, Fedichev P, Serganov A, Nudler E
Science 372 1169-1175 (2021)
PMID: 34112687

Function and Biology Details

Reactions catalysed: 
O(4)-succinyl-L-homoserine + L-cysteine = L-cystathionine + succinate
(1a) L-cystathionine = L-cysteine + 2-aminobut-2-enoate
L-cystathionine + H(2)O = L-homocysteine + NH(3) + pyruvate
Biochemical function:
Biological process:
Cellular component:
Sequence domain:

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-197860 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Cystathionine beta-lyase Chain: H
Molecule details ›
Chain: H
Length: 380 amino acids
Theoretical weight: 41.51 KDa
Source organism: Staphylococcus aureus
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: X5E0F1 (Residues: 1-380; Coverage: 100%)
Gene names: ACR79_13385, CNH36_02455, EIG94_05560, EIH03_00080, EP54_01605, EQ90_13355, GF599_08590, GO814_02345, GO942_04060, GQX37_00125, GZ130_10780, LB359_02510, NCTC10702_00840, NCTC13131_05913, SAMEA1029512_02290, SAMEA1029528_01119, SAMEA2078260_02460, SAMEA2078588_01369, SAMEA2080344_01429, SAMEA2081063_02563, SAMEA4008575_01592, SAMEA70146418_02721, mccB, mccB_2
Sequence domains: Cys/Met metabolism PLP-dependent enzyme

Ligands and Environments


Cofactor: Ligand PLP 1 x PLP
2 bound ligands:
Ligand NA 2 x NA
Ligand GOL 4 x GOL
1 modified residue:
Ligand LLP 1 x LLP

Experiments and Validation Details

wwPDB Validation report is not available for this entry.
X-ray source: NSLS-II BEAMLINE 17-ID-1
Spacegroup: I4122
Unit cell:
a: 105.133Å b: 105.133Å c: 288.148Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.163 0.162 0.184
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

17 review citations

Emerging roles of cystathionine β-synthase in various forms of cancer.
Ascenção et al. (2022)
16 more

1 mention without citation

Inhibitors of bacterial H2S biogenesis targeting antibiotic resistance and tolerance.
Shatalin et al. (2021)