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› 7mem

Electron Microscopy
3.2Å resolution

CryoEM structure of monoclonal Fab 045-09 2B05 binding the lateral patch of influenza virus H1 HA

Released:
DOI: 10.2210/pdb7mem/pdb
PDB entry 7mem coloured by chain, front view.
PDB entry 7mem coloured by chain, side view.
PDB entry 7mem coloured by chain, top view.
Source organisms:
Primary publication:
First exposure to the pandemic H1N1 virus induced broadly neutralizing antibodies targeting hemagglutinin head epitopes.
Guthmiller JJ, Han J, Li L, Freyn AW, Liu STH, Stovicek O, Stamper CT, Dugan HL, Tepora ME, Utset HA, Bitar DJ, Hamel NJ, Changrob S, Zheng NY, Huang M, Krammer F, Nachbagauer R, Palese P, Ward AB, Wilson PC
Sci Transl Med 13 (2021)
PMID: 34078743
Related structures: EMD-23792

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero dodecamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-207716 (preferred)
Entry contents:
4 distinct polypeptide molecules
Macromolecules (6 distinct):
Hemagglutinin Chains: C, E, F
Molecule details ›
Chains: C, E, F
Length: 331 amino acids
Theoretical weight: 36.73 KDa
Source organism: Influenza A virus (A/California/04/2009(H1N1))
Expression system: Homo sapiens
UniProt:
  • Canonical: C3W5S1 (Residues: 18-344; Coverage: 58%)
Gene name: HA
Sequence domains: Haemagglutinin
Light chain of monoclonal antibody 045-09 2B05 Chains: J, K, L
Molecule details ›
Chains: J, K, L
Length: 107 amino acids
Theoretical weight: 11.77 KDa
Source organism: Homo sapiens
Expression system: Homo sapiens
Heavy chain of monoclonal antibody 045-09 2B05 Chains: G, H, I
Hemagglutinin HA2 chain Chains: A, B, D

Ligands and Environments

Carbohydrate polymer : NEW Components: NAG, FUC
Carbohydrate polymer
Carbohydrate polymer : NEW Components: NAG
Carbohydrate polymer
1 bound ligand:
Ligand NAG 6 x NAG
No modified residues

Experiments and Validation Details

wwPDB Validation report is not available for this EM entry.
Resolution: 3.2Å
Relevant EMDB volumes: EMD-23792
Expression system: Homo sapiens

Quick links

Citations

3 review citations

Impact of antigenic evolution and original antigenic sin on SARS-CoV-2 immunity.
Aguilar-Bretones et al. (2023)
2 more

5 mentions without citation

Broadly neutralizing antibodies target a haemagglutinin anchor epitope.
Guthmiller et al. (2022)
4 more