› 7mfg

Electron Microscopy
3.87Å resolution

Cryo-EM structure of the VRC310 clinical trial, vaccine-elicited, human antibody 310-030-1D06 Fab in complex with an H1 NC99 HA trimer

Released:
DOI: 10.2210/pdb7mfg/pdb
PDB entry 7mfg coloured by chain, front view.
PDB entry 7mfg coloured by chain, side view.
PDB entry 7mfg coloured by chain, top view.
Source organisms:
Primary publication:
A single residue in influenza virus H2 hemagglutinin enhances the breadth of the B cell response elicited by H2 vaccination.
Andrews SF, Raab JE, Gorman J, Gillespie RA, Cheung CSF, Rawi R, Cominsky LY, Boyington JC, Creanga A, Shen CH, Harris DR, Olia AS, Nazzari AF, Zhou T, Houser KV, Chen GL, Mascola JR, Graham BS, Kanekiyo M, Ledgerwood JE, Kwong PD, McDermott AB
Nat Med 28 373-382 (2022)
PMID: 35115707
Related structures: EMD-23816

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero dodecamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-212419 (preferred)
Entry contents:
4 distinct polypeptide molecules
Macromolecules (5 distinct):
Hemagglutinin HA1 chain Chains: A, C, G
Molecule details ›
Chains: A, C, G
Length: 326 amino acids
Theoretical weight: 36.41 KDa
Source organism: Influenza A virus
Expression system: Homo sapiens
UniProt:
  • Canonical: Q6WG00 (Residues: 18-343; Coverage: 58%)
Gene name: HA
Sequence domains: Haemagglutinin
Hemagglutinin HA2 chain Chains: B, D, I
Molecule details ›
Chains: B, D, I
Length: 222 amino acids
Theoretical weight: 25.26 KDa
Source organism: Influenza A virus
Expression system: Homo sapiens
UniProt:
  • Canonical: Q6WG00 (Residues: 344-519; Coverage: 31%)
Gene name: HA
Sequence domains: Haemagglutinin
310-030-1D06 Heavy Chains: E, H, J
310-030-1D06 Light Chains: F, K, L

Ligands and Environments

Carbohydrate polymer : NEW Components: NAG, BMA
Carbohydrate polymer
1 bound ligand:
Ligand NAG 21 x NAG
No modified residues

Experiments and Validation Details

wwPDB Validation report is not available for this EM entry.
Resolution: 3.87Å
Relevant EMDB volumes: EMD-23816
Expression system: Homo sapiens

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Citations

1 review citation

Antibodies to combat viral infections: development strategies and progress.
Pantaleo et al. (2022)
11 other articles