› 7mi2

X-ray diffraction
1.4Å resolution

Seb1-G476S RNA binding domain

Released:
DOI: 10.2210/pdb7mi2/pdb
PDB entry 7mi2 coloured by chain, front view.
PDB entry 7mi2 coloured by chain, side view.
PDB entry 7mi2 coloured by chain, top view.
Source organism: Schizosaccharomyces pombe
Primary publication:
Genetic screen for suppression of transcriptional interference identifies a gain-of-function mutation in Pol2 termination factor Seb1.
Schwer B, Garg A, Jacewicz A, Shuman S
Proc Natl Acad Sci U S A 118 (2021)
PMID: 34389684

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-194099 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Rpb7-binding protein seb1 Chain: A
Molecule details ›
Chain: A
Length: 153 amino acids
Theoretical weight: 17.05 KDa
Source organism: Schizosaccharomyces pombe
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q9UTE3 (Residues: 388-539; Coverage: 25%)
Gene names: SPAC222.09, seb1
Sequence domains:

Ligands and Environments

2 bound ligands:
Ligand GOL 1 x GOL
Ligand PEG 1 x PEG
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 24-ID-E
Spacegroup: C2
Unit cell:
a: 111.649Å b: 46.307Å c: 32.296Å
α: 90° β: 99.005° γ: 90°
R-values:
R R work R free
0.172 0.171 0.188
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

6 citation in other articles

Cleavage-Polyadenylation Factor Cft1 and SPX Domain Proteins Are Agents of Inositol Pyrophosphate Toxicosis in Fission Yeast.
Schwer et al. (2022)
5 more