› 7mju

X-ray diffraction
2.1Å resolution

Crystal structure of human AF10 PZP bound to histone H3 tail

Released:
DOI: 10.2210/pdb7mju/pdb
PDB entry 7mju coloured by chain, front view.
PDB entry 7mju coloured by chain, side view.
PDB entry 7mju coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
The role of the PZP domain of AF10 in acute leukemia driven by AF10 translocations.
Klein BJ, Deshpande A, Cox KL, Xuan F, Zandian M, Barbosa K, Khanal S, Tong Q, Zhang Y, Zhang P, Sinha A, Bohlander SK, Shi X, Wen H, Poirier MG, Deshpande AJ, Kutateladze TG
Nat Commun 12 4130 (2021)
PMID: 34226546

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-157212 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Histone H3.1; Protein AF-10 Chain: A
Molecule details ›
Chain: A
Length: 206 amino acids
Theoretical weight: 22.61 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli K-12
UniProt:
  • Canonical: P68431 (Residues: 2-14; Coverage: 10%)
  • Canonical: P55197 (Residues: 19-208; Coverage: 18%)
Gene names: AF10, H3C1, H3C10, H3C11, H3C12, H3C2, H3C3, H3C4, H3C6, H3C7, H3C8, H3FA, H3FB, H3FC HIST1H3C, H3FD, H3FF, H3FH, H3FI, H3FJ, H3FK, H3FL, HIST1H3A, HIST1H3B, HIST1H3D, HIST1H3E, HIST1H3F, HIST1H3G, HIST1H3H, HIST1H3I, HIST1H3J, MLLT10
Sequence domains:

Ligands and Environments

1 bound ligand:
Ligand ZN 5 x ZN
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ALS BEAMLINE 4.2.2
Spacegroup: P21
Unit cell:
a: 42.92Å b: 54.33Å c: 47.78Å
α: 90° β: 106.82° γ: 90°
R-values:
R R work R free
0.167 0.165 0.206
Expression system: Escherichia coli K-12

Quick links

Citations

1 review citation

Non-histone binding functions of PHD fingers.
Gaurav et al. (2023)
5 other articles

1 mention without citation

Atypical histone targets of PHD fingers.
Black et al. (2023)