› 7mna

X-ray diffraction
1.47Å resolution

PTP1B 1-284 F225Y-R199N in complex with TCS401

Released:
DOI: 10.2210/pdb7mna/pdb
PDB entry 7mna coloured by chain, front view.
PDB entry 7mna coloured by chain, side view.
PDB entry 7mna coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Conserved conformational dynamics determine enzyme activity.
Torgeson KR, Clarkson MW, Granata D, Lindorff-Larsen K, Page R, Peti W
Sci Adv 8 eabo5546 (2022)
PMID: 35921420

Function and Biology Details

Reaction catalysed: 
Protein tyrosine phosphate + H(2)O = protein tyrosine + phosphate
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-148259 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Tyrosine-protein phosphatase non-receptor type 1 Chain: A
Molecule details ›
Chain: A
Length: 289 amino acids
Theoretical weight: 33.51 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P18031 (Residues: 1-284; Coverage: 65%)
Gene names: PTP1B, PTPN1
Sequence domains: Protein-tyrosine phosphatase

Ligands and Environments

4 bound ligands:
Ligand OTA 1 x OTA
Ligand GOL 1 x GOL
Ligand CL 4 x CL
Ligand TRS 1 x TRS
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRL BEAMLINE BL12-2
Spacegroup: P3221
Unit cell:
a: 88.004Å b: 88.004Å c: 72.282Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.171 0.17 0.194
Expression system: Escherichia coli

Quick links

Citations

1 review citation

Loop dynamics and the evolution of enzyme activity.
Corbella et al. (2023)
9 other articles

1 mention without citation

Native dynamics and allosteric responses in PTP1B probed by high-resolution HDX-MS.
Woods et al. (2023)