› 7msk

X-ray diffraction
2.06Å resolution

ThuS glycosin S-glycosyltransferase

Released:
DOI: 10.2210/pdb7msk/pdb
PDB entry 7msk coloured by chain, front view.
PDB entry 7msk coloured by chain, side view.
PDB entry 7msk coloured by chain, top view.
Source organism: Bacillus thuringiensis serovar andalousiensis BGSC 4AW1
Primary publication:
Structural and mechanistic investigations of protein S-glycosyltransferases.
Fujinami D, Garcia de Gonzalo CV, Biswas S, Hao Y, Wang H, Garg N, Lukk T, Nair SK, van der Donk WA
Cell Chem Biol 28 1740-1749.e6 (2021)
PMID: 34283964

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-111155 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Glycosyltransferase 2-like domain-containing protein Chains: A, B
Molecule details ›
Chains: A, B
Length: 430 amino acids
Theoretical weight: 50.45 KDa
Source organism: Bacillus thuringiensis serovar andalousiensis BGSC 4AW1
Expression system: Escherichia coli
UniProt:
  • Canonical: C3GCL5 (Residues: 1-424; Coverage: 100%)
Gene name: bthur0009_56280
Sequence domains: Glycosyl transferase family 2

Ligands and Environments

2 bound ligands:
Ligand MG 2 x MG
Ligand U2F 2 x U2F
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 21-ID-G
Spacegroup: P21
Unit cell:
a: 49.833Å b: 112.654Å c: 90.31Å
α: 90° β: 90.11° γ: 90°
R-values:
R R work R free
0.202 0.2 0.239
Expression system: Escherichia coli

Quick links

Citations

2 review citations

Emulating nonribosomal peptides with ribosomal biosynthetic strategies.
Mordhorst et al. (2023)
1 more

1 mention without citation

Structural and mechanistic investigations of protein S-glycosyltransferases.
Fujinami et al. (2021)