› 7myg

X-ray diffraction
2.51Å resolution

Function and Biology Details

Reactions catalysed: 
2 alpha,alpha'-trehalose 6-mycolate = alpha,alpha'-trehalose + alpha,alpha'-trehalose 6,6'-bismycolate
Acyl-CoA + 1,2-diacylglycerol = CoA + triacylglycerol
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-162087 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Diacylglycerol acyltransferase/mycolyltransferase Ag85C Chains: A, B
Molecule details ›
Chains: A, B
Length: 278 amino acids
Theoretical weight: 30.59 KDa
Source organism: Mycobacterium tuberculosis
Expression system: Escherichia coli
UniProt:
  • Canonical: P9WQN9 (Residues: 51-328; Coverage: 94%)
Gene names: MTCI5.03c, Rv0129c, fbpC, mpt45
Sequence domains: Putative esterase

Ligands and Environments

1 bound ligand:
Ligand GOL 14 x GOL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 21-ID-F
Spacegroup: P21
Unit cell:
a: 59.458Å b: 70.606Å c: 68.59Å
α: 90° β: 89.97° γ: 90°
R-values:
R R work R free
0.162 0.156 0.222
Expression system: Escherichia coli

Quick links

Citations

1 review citation

Activity-based probes in pathogenic bacteria: Investigating drug targets and molecule specificity.
Lembke et al. (2023)