› 7n0i

X-ray diffraction
2.2Å resolution

Structure of the SARS-CoV-2 N protein C-terminal domain bound to single-domain antibody E2

Released:
DOI: 10.2210/pdb7n0i/pdb
Model geometry
Fit model/data
PDB entry 7n0i coloured by chain, front view.
PDB entry 7n0i coloured by chain, side view.
PDB entry 7n0i coloured by chain, top view.
Source organisms:
Primary publication:
Structural Basis for SARS-CoV-2 Nucleocapsid Protein Recognition by Single-Domain Antibodies.
Ye Q, Lu S, Corbett KD
OpenAccess logo Front Immunol 12 719037 (2021)
PMID: 34381460

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-210454 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Nucleoprotein Chains: A, B, C, D, E, F, G, H
Molecule details ›
Chains: A, B, C, D, E, F, G, H
Length: 96 amino acids
Theoretical weight: 10.82 KDa
Source organism: Severe acute respiratory syndrome coronavirus 2
Expression system: Escherichia coli
UniProt:
  • Canonical: P0DTC9 (Residues: 269-364; Coverage: 23%)
Gene name: N
Single-domain antibody E2 Chains: I, J, K, L
Molecule details ›
Chains: I, J, K, L
Length: 139 amino acids
Theoretical weight: 15.43 KDa
Source organism: Lama glama
Expression system: Escherichia coli

Ligands and Environments

2 bound ligands:
Ligand ACT 2 x ACT
Ligand MG 1 x MG
No modified residues

Experiments and Validation Details

wwPDB Validation report is not available for this entry.
X-ray source: ALS BEAMLINE 5.0.2
Spacegroup: P212121
Unit cell:
a: 75.846Å b: 131.557Å c: 140.048Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.241 0.239 0.271
Expression system: Escherichia coli

Quick links

Citations

3 review citations

Liquid-liquid phase separation of nucleocapsid proteins during SARS-CoV-2 and HIV-1 replication.
Chau et al. (2023)
2 more

5 mentions without citation

Structural Basis for SARS-CoV-2 Nucleocapsid Protein Recognition by Single-Domain Antibodies.
Ye et al. (2021)
4 more