› 7n7g

X-ray diffraction
2Å resolution

Crystal Structure of FosB from Enterococcus faecium with Fosfomycin

Released:
DOI: 10.2210/pdb7n7g/pdb
PDB entry 7n7g coloured by chain, front view.
PDB entry 7n7g coloured by chain, side view.
PDB entry 7n7g coloured by chain, top view.
Source organism: Enterococcus faecium
Primary publication:
Structural and functional characterization of fosfomycin resistance conferred by FosB from Enterococcus faecium.
Wiltsie V, Travis S, Shay MR, Simmons Z, Frantom P, Thompson MK
Protein Sci 31 580-590 (2022)
PMID: 34882867

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-123283 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
VOC domain-containing protein Chains: A, B
Molecule details ›
Chains: A, B
Length: 139 amino acids
Theoretical weight: 16.44 KDa
Source organism: Enterococcus faecium
Expression system: Escherichia coli
UniProt:
  • Canonical: F1C939 (Residues: 1-139; Coverage: 100%)
Gene name: fosB
Sequence domains: Glyoxalase/Bleomycin resistance protein/Dioxygenase superfamily

Ligands and Environments

3 bound ligands:
Ligand MN 2 x MN
Ligand EDO 6 x EDO
Ligand FCN 2 x FCN
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU
Spacegroup: P41212
Unit cell:
a: 79.874Å b: 79.874Å c: 95.639Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.216 0.214 0.254
Expression system: Escherichia coli

Quick links

Citations

3 citation in other articles

Identification and analysis of small molecule inhibitors of FosB from Staphylococcus aureus.
Travis et al. (2023)
2 more

1 mention without citation

Structural and functional characterization of fosfomycin resistance conferred by FosB from Enterococcus faecium.
Wiltsie et al. (2022)