› 7na9

X-ray diffraction
1.76Å resolution

Crystal structure of BoNT/B-LC-JSG-C1

Released:
DOI: 10.2210/pdb7na9/pdb
Model geometry
Fit model/data
PDB entry 7na9 coloured by chain, front view.
PDB entry 7na9 coloured by chain, side view.
PDB entry 7na9 coloured by chain, top view.
Source organisms:
Primary publication:
Probing the structure and function of the protease domain of botulinum neurotoxins using single-domain antibodies.
Lam KH, Tremblay JM, Perry K, Ichtchenko K, Shoemaker CB, Jin R
OpenAccess logo PLoS Pathog 18 e1010169 (2022)
PMID: 34990480

Function and Biology Details

Reaction catalysed: 
Limited hydrolysis of proteins of the neuroexocytosis apparatus,synaptobrevins, SNAP25 or syntaxin. No detected action on small moleculesubstrates.
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-210497 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Botulinum neurotoxin B light chain Chain: A
Molecule details ›
Chain: A
Length: 446 amino acids
Theoretical weight: 51.45 KDa
Source organism: Clostridium botulinum
Expression system: Escherichia coli
UniProt:
  • Canonical: P10844 (Residues: 1-441; Coverage: 34%)
Gene name: botB
Sequence domains: Clostridial neurotoxin zinc protease
JSG-C1 Chain: D
Molecule details ›
Chain: D
Length: 134 amino acids
Theoretical weight: 14.47 KDa
Source organism: Vicugna pacos
Expression system: Escherichia coli

Ligands and Environments

2 bound ligands:
Ligand EDO 3 x EDO
Ligand ZN 1 x ZN
No modified residues

Experiments and Validation Details

wwPDB Validation report is not available for this entry.
X-ray source: APS BEAMLINE 24-ID-E
Spacegroup: P212121
Unit cell:
a: 61.417Å b: 97.018Å c: 101.904Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.169 0.167 0.205
Expression system: Escherichia coli

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Citations

1 review citation

Single Domain Antibody application in bacterial infection diagnosis and neutralization.
Qin et al. (2022)
7 other articles