› 7nal

Electron Microscopy
3Å resolution

Cryo-EM structure of activated human SARM1 in complex with NMN and 1AD (ARM and SAM domains)

Released:
DOI: 10.2210/pdb7nal/pdb
PDB entry 7nal coloured by chain, front view.
PDB entry 7nal coloured by chain, side view.
PDB entry 7nal coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Structural basis of SARM1 activation, substrate recognition, and inhibition by small molecules.
Shi Y, Kerry PS, Nanson JD, Bosanac T, Sasaki Y, Krauss R, Saikot FK, Adams SE, Mosaiab T, Masic V, Mao X, Rose F, Vasquez E, Furrer M, Cunnea K, Brearley A, Gu W, Luo Z, Brillault L, Landsberg MJ, DiAntonio A, Kobe B, Milbrandt J, Hughes RO, Ve T
Mol Cell 82 1643-1659.e10 (2022)
PMID: 35334231
Related structures: EMD-24273

Function and Biology Details

Reaction catalysed: 
(1a) NAD(+) = cyclic ADP-ribose + nicotinamide
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo octamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-180347 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
NAD(+) hydrolase SARM1 Chains: A, B, C, D, E, F, G, H
Molecule details ›
Chains: A, B, C, D, E, F, G, H
Length: 697 amino acids
Theoretical weight: 76.47 KDa
Source organism: Homo sapiens
Expression system: Homo sapiens
UniProt:
  • Canonical: Q6SZW1 (Residues: 28-724; Coverage: 96%)
Gene names: KIAA0524, SAMD2, SARM, SARM1
Sequence domains:

Ligands and Environments

1 bound ligand:
Ligand NMN 8 x NMN
No modified residues

Experiments and Validation Details

wwPDB Validation report is not available for this EM entry.
Resolution: 3Å
Relevant EMDB volumes: EMD-24273
Expression system: Homo sapiens

Quick links

Citations

6 review citations

The chemical biology of NAD+ regulation in axon degeneration.
Icso et al. (2022)
5 more

2 mentions without citation

Structural basis of SARM1 activation, substrate recognition, and inhibition by small molecules.
Shi et al. (2022)
1 more