› 7nb5

X-ray diffraction
2.13Å resolution

Structure of EstD11 S144A in complex with naproxen p-nitrophenol ester

Released:
DOI: 10.2210/pdb7nb5/pdb
PDB entry 7nb5 coloured by chain, front view.
PDB entry 7nb5 coloured by chain, side view.
PDB entry 7nb5 coloured by chain, top view.
Source organism: uncultured bacterium
Primary publication:
Biochemical and Structural Characterization of a novel thermophilic esterase EstD11 provide catalytic insights for the HSL family.
Miguel-Ruano V, Rivera I, Rajkovic J, Knapik K, Torrado A, Otero JM, Beneventi E, Becerra M, Sánchez-Costa M, Hidalgo A, Berenguer J, González-Siso MI, Cruces J, Rúa ML, Hermoso JA
Comput Struct Biotechnol J 19 1214-1232 (2021)
PMID: 33680362

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-168525 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
EstD11 S144A Chain: A
Molecule details ›
Chain: A
Length: 295 amino acids
Theoretical weight: 32.04 KDa
Source organism: uncultured bacterium
Expression system: Escherichia coli BL21(DE3)

Ligands and Environments

1 bound ligand:
Ligand U68 1 x U68
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ALBA BEAMLINE XALOC
Spacegroup: I222
Unit cell:
a: 47.999Å b: 89.705Å c: 125.762Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.17 0.167 0.228
Expression system: Escherichia coli BL21(DE3)

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Citations

8 citation in other articles

Identification and Biochemical Characterization of a Novel Hormone-Sensitive Lipase Family Esterase Est19 from the Antarctic Bacterium Pseudomonas sp. E2-15.
Liu et al. (2021)
7 more

1 mention without citation

Enzymatic Characterization of a Novel HSL Family IV Esterase EstD04 from Pseudomonas sp. D01 in Mealworm Gut Microbiota.
Kuan et al. (2023)