› 7ncg

Electron Microscopy
3.43Å resolution

Type 2A alpha-synuclein filament seeded in vitro by filaments purified from Multiple Systems Atrophy Case 2

Released:
DOI: 10.2210/pdb7ncg/pdb
PDB entry 7ncg coloured by chain, front view.
PDB entry 7ncg coloured by chain, side view.
PDB entry 7ncg coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Seeded assembly in vitro does not replicate the structures of α-synuclein filaments from multiple system atrophy.
Lövestam S, Schweighauser M, Matsubara T, Murayama S, Tomita T, Ando T, Hasegawa K, Yoshida M, Tarutani A, Hasegawa M, Goedert M, Scheres SHW
FEBS Open Bio 11 999-1013 (2021)
PMID: 33548114
Related structures: EMD-12265

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo hexamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-153511 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Alpha-synuclein Chains: A, B, C, D, E, F, G, H, I, J, K, L
Molecule details ›
Chains: A, B, C, D, E, F, G, H, I, J, K, L
Length: 140 amino acids
Theoretical weight: 14.48 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P37840 (Residues: 1-140; Coverage: 100%)
Gene names: NACP, PARK1, SNCA
Sequence domains: Synuclein

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 3.43Å
Relevant EMDB volumes: EMD-12265
Expression system: Escherichia coli

Quick links

Citations

20 review citations

Neuropathology and molecular diagnosis of Synucleinopathies.
Koga et al. (2021)
19 more

3 mentions without citation

Seeded assembly in vitro does not replicate the structures of α-synuclein filaments from multiple system atrophy.
Lövestam et al. (2021)
2 more