› 7nes

X-ray diffraction
1.35Å resolution

Crystal structure of the v-Src SH3 domain N117D-V124L mutant

Released:
DOI: 10.2210/pdb7nes/pdb
PDB entry 7nes coloured by chain, front view.
PDB entry 7nes coloured by chain, side view.
PDB entry 7nes coloured by chain, top view.
Source organism: Gallus gallus
Primary publication:
The impact of oncogenic mutations of the viral Src kinase on the structure and stability of the SH3 domain.
Salinas-Garcia MC, Plaza-Garrido M, Camara-Artigas A
Acta Crystallogr D Struct Biol 77 854-866 (2021)
PMID: 34076598

Function and Biology Details

Reaction catalysed: 
ATP + a [protein]-L-tyrosine = ADP + a [protein]-L-tyrosine phosphate
Biochemical function:
  • not assigned
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-132629 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Proto-oncogene tyrosine-protein kinase Src Chain: A
Molecule details ›
Chain: A
Length: 61 amino acids
Theoretical weight: 6.85 KDa
Source organism: Gallus gallus
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P00523 (Residues: 81-141; Coverage: 11%)
Gene name: SRC
Sequence domains: SH3 domain

Ligands and Environments

1 bound ligand:
Ligand GLY 1 x GLY
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID30B
Spacegroup: P3221
Unit cell:
a: 37.171Å b: 37.171Å c: 65.824Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.159 0.158 0.183
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

2 review citations

Heat Shock Protein 90 (HSP90) Inhibitors as Anticancer Medicines: A Review on the Computer-Aided Drug Discovery Approaches over the Past Five Years.
Magwenyane et al. (2022)
1 more

1 mention without citation

The impact of oncogenic mutations of the viral Src kinase on the structure and stability of the SH3 domain.
Salinas-Garcia et al. (2021)