› 7nfz

X-ray diffraction
1.55Å resolution

Crystal structure of haloalkane dehalogenase LinB57 mutant (H272F) from Sphingobium japonicum UT26

Released:
DOI: 10.2210/pdb7nfz/pdb
PDB entry 7nfz coloured by chain, front view.
PDB entry 7nfz coloured by chain, side view.
PDB entry 7nfz coloured by chain, top view.
Source organism: Sphingobium japonicum UT26S
Entry author: Marek M

Function and Biology Details

Reaction catalysed: 
1-haloalkane + H(2)O = a primary alcohol + halide
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-112655 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Haloalkane dehalogenase Chain: A
Molecule details ›
Chain: A
Length: 302 amino acids
Theoretical weight: 33.98 KDa
Source organism: Sphingobium japonicum UT26S
Expression system: Escherichia coli
UniProt:
  • Canonical: D4Z2G1 (Residues: 1-296; Coverage: 100%)
Gene names: SJA_C1-19590, linB
Sequence domains: alpha/beta hydrolase fold

Ligands and Environments

5 bound ligands:
Ligand CXS 4 x CXS
Ligand GOL 3 x GOL
Ligand TRS 1 x TRS
Ligand PO4 1 x PO4
Ligand K 1 x K
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SLS BEAMLINE X06DA
Spacegroup: P212121
Unit cell:
a: 51.496Å b: 65.448Å c: 90.171Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.166 0.164 0.19
Expression system: Escherichia coli

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