› 7nn1

X-ray diffraction
1.54Å resolution

Crystal structure of Mycobacterium tuberculosis ArgD with prosthetic group pyridoxal 5'-phosphate

Released:
DOI: 10.2210/pdb7nn1/pdb
PDB entry 7nn1 coloured by chain, front view.
PDB entry 7nn1 coloured by chain, side view.
PDB entry 7nn1 coloured by chain, top view.
Source organism: Mycobacterium tuberculosis H37Rv
Primary publication:
A fragment-based approach to assess the ligandability of ArgB, ArgC, ArgD and ArgF in the L-arginine biosynthetic pathway of Mycobacterium tuberculosis
Gupta P, Thomas SE, Zaidan SA, Pasillas MA, Cory-Wright J, Sebastian-Perez V, Burgess A, Cattermole E, Meghir C, Abell C, Coyne AG, Jacobs WR, Blundell TL, Tiwari S, Mendes V
Comput Struct Biotechnol J 19 3491-3506 (2021)

Function and Biology Details

Reaction catalysed: 
N(2)-acetyl-L-ornithine + 2-oxoglutarate = N-acetyl-L-glutamate 5-semialdehyde + L-glutamate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-162034 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Acetylornithine aminotransferase Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 402 amino acids
Theoretical weight: 41.33 KDa
Source organism: Mycobacterium tuberculosis H37Rv
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P9WPZ7 (Residues: 1-400; Coverage: 100%)
Gene names: MTCY06H11.20, Rv1655, argD
Sequence domains: Aminotransferase class-III

Ligands and Environments

1 bound ligand:
Ligand NO3 4 x NO3
1 modified residue:
Ligand LLP 4 x LLP

Experiments and Validation Details

Entry percentile scores
X-ray source: DIAMOND BEAMLINE I03
Spacegroup: P21
Unit cell:
a: 65.055Å b: 184.265Å c: 71.545Å
α: 90° β: 106.1° γ: 90°
R-values:
R R work R free
0.178 0.177 0.199
Expression system: Escherichia coli BL21(DE3)

Quick links