› 7ntl

X-ray diffraction
1.38Å resolution

AA9 lytic polysaccharide monooxygenase (LPMO) from Malbranchea cinnamomea

Released:
DOI: 10.2210/pdb7ntl/pdb
PDB entry 7ntl coloured by chain, front view.
PDB entry 7ntl coloured by chain, side view.
PDB entry 7ntl coloured by chain, top view.
Source organism: Malbranchea cinnamomea
Primary publication:
Structure of a C1/C4-oxidizing AA9 lytic polysaccharide monooxygenase from the thermophilic fungus Malbranchea cinnamomea.
Mazurkewich S, Seveso A, Hüttner S, Brändén G, Larsbrink J
Acta Crystallogr D Struct Biol 77 1019-1026 (2021)
PMID: 34342275

Function and Biology Details

Reaction catalysed: 
Endohydrolysis of (1->4)-beta-D-glucosidic linkages in cellulose, lichenin and cereal beta-D-glucans
Biochemical function:
Biological process:
Cellular component:
Sequence domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-105422 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
AA9 family lytic polysaccharide monooxygenase F Chain: A
Molecule details ›
Chain: A
Length: 222 amino acids
Theoretical weight: 23.23 KDa
Source organism: Malbranchea cinnamomea
Expression system: Komagataella pastoris
UniProt:
  • Canonical: A0A5J6BJN2 (Residues: 21-242; Coverage: 100%)
Gene names: AA9F, LPMO9F
Sequence domains: Auxiliary Activity family 9 (formerly GH61)

Ligands and Environments

2 bound ligands:
Ligand CU 1 x CU
Ligand CIT 1 x CIT
1 modified residue:
Ligand SNN 1 x SNN

Experiments and Validation Details

Entry percentile scores
X-ray source: MAX IV BEAMLINE BioMAX
Spacegroup: P21
Unit cell:
a: 38.956Å b: 43.128Å c: 52.341Å
α: 90° β: 101.814° γ: 90°
R-values:
R R work R free
0.163 0.161 0.193
Expression system: Komagataella pastoris

Quick links

Citations

1 mention without citation

Structure of a C1/C4-oxidizing AA9 lytic polysaccharide monooxygenase from the thermophilic fungus Malbranchea cinnamomea.
Mazurkewich et al. (2021)