› 7nwg

Electron Microscopy
3.8Å resolution

Mammalian pre-termination 80S ribosome with Hybrid P/E- and A/P-site tRNA's bound by Blasticidin S.

Released:
DOI: 10.2210/pdb7nwg/pdb
PDB entry 7nwg coloured by chain, front view.
PDB entry 7nwg coloured by chain, side view.
PDB entry 7nwg coloured by chain, top view.
Source organism: Oryctolagus cuniculus
Primary publication:
Blasticidin S inhibits mammalian translation and enhances production of protein encoded by nonsense mRNA.
Powers KT, Stevenson-Jones F, Yadav SKN, Amthor B, Bufton JC, Borucu U, Shen D, Becker JP, Lavysh D, Hentze MW, Kulozik AE, Neu-Yilik G, Schaffitzel C
Nucleic Acids Res 49 7665-7679 (2021)
PMID: 34157102
Related structures: EMD-12631

Function and Biology Details

Reaction catalysed: 
The C-O-P bond 3' to the apurinic or apyrimidinic site in DNA is broken by a beta-elimination reaction, leaving a 3'-terminal unsaturated sugar and a product with a terminal 5'-phosphate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero 86-mer (preferred)
PDBe Complex ID:
PDB-CPX-243229 (preferred)
Entry contents:
79 distinct polypeptide molecules
7 distinct RNA molecules
Macromolecules (86 distinct):
Small ribosomal subunit protein uS2 Chain: B2
Molecule details ›
Chain: B2
Length: 295 amino acids
Theoretical weight: 32.96 KDa
Source organism: Oryctolagus cuniculus
UniProt:
  • Canonical: G1TLT8 (Residues: 1-295; Coverage: 100%)
Gene name: RPSA
Sequence domains:
Small ribosomal subunit protein eS1 Chain: C2
Molecule details ›
Chain: C2
Length: 264 amino acids
Theoretical weight: 29.94 KDa
Source organism: Oryctolagus cuniculus
UniProt:
  • Canonical: G1SS70 (Residues: 1-264; Coverage: 100%)
Gene name: RPS3A
Sequence domains: Ribosomal S3Ae family
40S ribosomal protein S2 Chain: D2
Molecule details ›
Chain: D2
Length: 259 amino acids
Theoretical weight: 28 KDa
Source organism: Oryctolagus cuniculus
Small ribosomal subunit protein uS3 Chain: E2
Molecule details ›
Chain: E2
Length: 281 amino acids
Theoretical weight: 31.15 KDa
Source organism: Oryctolagus cuniculus
UniProt:
  • Canonical: G1TNM3 (Residues: 1-243; Coverage: 100%)
Gene name: RPS3
Sequence domains:
S4 Chain: F2
Molecule details ›
Chain: F2
Length: 262 amino acids
Theoretical weight: 29.52 KDa
Source organism: Oryctolagus cuniculus
Small ribosomal subunit protein uS7 Chain: G2
Molecule details ›
Chain: G2
Length: 205 amino acids
Theoretical weight: 23.04 KDa
Source organism: Oryctolagus cuniculus
UniProt:
  • Canonical: G1TFM5 (Residues: 1-204; Coverage: 100%)
Gene name: RPS5
Sequence domains: Ribosomal protein S7p/S5e
40S ribosomal protein S6 Chain: H2
Molecule details ›
Chain: H2
Length: 262 amino acids
Theoretical weight: 30.07 KDa
Source organism: Oryctolagus cuniculus
UniProt:
  • Canonical: A0A5K1UJS7 (Residues: 1-249; Coverage: 100%)
Sequence domains: Ribosomal protein S6e
Small ribosomal subunit protein eS7 Chain: I2
Molecule details ›
Chain: I2
Length: 190 amino acids
Theoretical weight: 21.72 KDa
Source organism: Oryctolagus cuniculus
UniProt:
  • Canonical: G1SVB0 (Residues: 5-194; Coverage: 98%)
Gene name: RPS7
Small ribosomal subunit protein eS8 Chain: J2
Molecule details ›
Chain: J2
Length: 206 amino acids
Theoretical weight: 24 KDa
Source organism: Oryctolagus cuniculus
UniProt:
  • Canonical: G1TJW1 (Residues: 2-207; Coverage: 99%)
Gene name: RPS8
Sequence domains: Ribosomal protein S8e
Small ribosomal subunit protein uS4 Chain: K2
Molecule details ›
Chain: K2
Length: 194 amino acids
Theoretical weight: 22.66 KDa
Source organism: Oryctolagus cuniculus
UniProt:
  • Canonical: A0A1S3APZ1 (Residues: 1-194; Coverage: 100%)
Gene name: RPS9
Sequence domains:
Small ribosomal subunit protein eS10 Chain: L2
Molecule details ›
Chain: L2
Length: 151 amino acids
Theoretical weight: 17.42 KDa
Source organism: Oryctolagus cuniculus
UniProt:
  • Canonical: A0A2F0BDG8 (Residues: 58-208; Coverage: 68%)
Gene name: ESR_09716
Sequence domains: Plectin/S10 domain
Small ribosomal subunit protein uS17 Chain: M2
Molecule details ›
Chain: M2
Length: 158 amino acids
Theoretical weight: 18.51 KDa
Source organism: Oryctolagus cuniculus
UniProt:
  • Canonical: G1TRM4 (Residues: 1-158; Coverage: 100%)
Gene name: RPS11
Sequence domains:
Small ribosomal subunit protein eS12 Chain: N2
Molecule details ›
Chain: N2
Length: 123 amino acids
Theoretical weight: 13.71 KDa
Source organism: Oryctolagus cuniculus
UniProt:
  • Canonical: G1SFR8 (Residues: 10-132; Coverage: 93%)
Gene name: RPS12
Sequence domains: Ribosomal protein L7Ae/L30e/S12e/Gadd45 family
Small ribosomal subunit protein uS15 Chain: O2
Molecule details ›
Chain: O2
Length: 149 amino acids
Theoretical weight: 17.06 KDa
Source organism: Oryctolagus cuniculus
UniProt:
  • Canonical: G1SP51 (Residues: 2-150; Coverage: 99%)
Gene name: RPS13
Sequence domains:
Small ribosomal subunit protein uS11 Chain: P2
Molecule details ›
Chain: P2
Length: 168 amino acids
Theoretical weight: 18.13 KDa
Source organism: Oryctolagus cuniculus
UniProt:
  • Canonical: G1T1F0 (Residues: 1-151; Coverage: 100%)
Gene name: RPS14
Sequence domains: Ribosomal protein S11
Small ribosomal subunit protein uS19 Chain: Q2
Molecule details ›
Chain: Q2
Length: 145 amino acids
Theoretical weight: 17.05 KDa
Source organism: Oryctolagus cuniculus
UniProt:
  • Canonical: G1U0Q2 (Residues: 1-145; Coverage: 100%)
Gene name: RPS15
Sequence domains: Ribosomal protein S19
Small ribosomal subunit protein uS9 Chain: R2
Molecule details ›
Chain: R2
Length: 157 amino acids
Theoretical weight: 17.59 KDa
Source organism: Oryctolagus cuniculus
UniProt:
  • Canonical: G1SGX4 (Residues: 1-146; Coverage: 100%)
Gene name: RPS16
Sequence domains: Ribosomal protein S9/S16
Small ribosomal subunit protein eS17 Chain: S2
Molecule details ›
Chain: S2
Length: 145 amino acids
Theoretical weight: 16.78 KDa
Source organism: Oryctolagus cuniculus
UniProt:
  • Canonical: S7Q348 (Residues: 1-145; Coverage: 100%)
Gene name: D623_10013203
Sequence domains: Ribosomal S17
Small ribosomal subunit protein uS13 Chain: T2
Molecule details ›
Chain: T2
Length: 152 amino acids
Theoretical weight: 17.76 KDa
Source organism: Oryctolagus cuniculus
UniProt:
  • Canonical: G1TPG3 (Residues: 1-152; Coverage: 100%)
Gene name: RPS18
Sequence domains: Ribosomal protein S13/S18
Small ribosomal subunit protein eS19 Chain: U2
Molecule details ›
Chain: U2
Length: 145 amino acids
Theoretical weight: 16.11 KDa
Source organism: Oryctolagus cuniculus
UniProt:
  • Canonical: G1TN62 (Residues: 1-145; Coverage: 100%)
Gene name: RPS19
Sequence domains: Ribosomal protein S19e
40S ribosomal protein S20 Chain: V2
Molecule details ›
Chain: V2
Length: 130 amino acids
Theoretical weight: 14.6 KDa
Source organism: Oryctolagus cuniculus
Small ribosomal subunit protein eS21 Chain: W2
Molecule details ›
Chain: W2
Length: 83 amino acids
Theoretical weight: 9.12 KDa
Source organism: Oryctolagus cuniculus
UniProt:
  • Canonical: Q32PB8 (Residues: 1-83; Coverage: 100%)
Gene name: RPS21
Sequence domains: Ribosomal protein S21e
Ribosomal protein S15a Chain: X2
Molecule details ›
Chain: X2
Length: 139 amino acids
Theoretical weight: 15.78 KDa
Source organism: Oryctolagus cuniculus
Small ribosomal subunit protein uS12 Chain: Y2
Molecule details ›
Chain: Y2
Length: 141 amino acids
Theoretical weight: 15.63 KDa
Source organism: Oryctolagus cuniculus
UniProt:
  • Canonical: G1SZ47 (Residues: 2-142; Coverage: 99%)
Gene name: RPS23
Sequence domains: Ribosomal protein S12/S23
40S ribosomal protein S24 Chain: Z2
Molecule details ›
Chain: Z2
Length: 146 amino acids
Theoretical weight: 17.01 KDa
Source organism: Oryctolagus cuniculus
ribosomal protein eS25 Chain: a2
Molecule details ›
Chain: a2
Length: 198 amino acids
Theoretical weight: 22.09 KDa
Source organism: Oryctolagus cuniculus
40S ribosomal protein S26 Chain: b2
Molecule details ›
Chain: b2
Length: 117 amino acids
Theoretical weight: 13.15 KDa
Source organism: Oryctolagus cuniculus
UniProt:
  • Canonical: T0M5X4 (Residues: 1-117; Coverage: 100%)
Gene name: CB1_000449017
Sequence domains: Ribosomal protein S26e
Small ribosomal subunit protein eS27 Chain: c2
Molecule details ›
Chain: c2
Length: 84 amino acids
Theoretical weight: 9.48 KDa
Source organism: Oryctolagus cuniculus
UniProt:
  • Canonical: G1TZ76 (Residues: 1-84; Coverage: 100%)
Gene name: RPS27
Sequence domains: Ribosomal protein S27
Small ribosomal subunit protein eS28 Chain: d2
Molecule details ›
Chain: d2
Length: 69 amino acids
Theoretical weight: 7.84 KDa
Source organism: Oryctolagus cuniculus
UniProt:
  • Canonical: G1TIB4 (Residues: 1-69; Coverage: 100%)
Gene name: RPS28
Sequence domains: Ribosomal protein S28e
Small ribosomal subunit protein uS14 Chain: e2
Molecule details ›
Chain: e2
Length: 55 amino acids
Theoretical weight: 6.56 KDa
Source organism: Oryctolagus cuniculus
UniProt:
  • Canonical: G1U7M4 (Residues: 2-56; Coverage: 98%)
Gene name: RPS29
Sequence domains: Ribosomal protein S14p/S29e
Ubiquitin-like FUBI-ribosomal protein eS30 fusion protein Chain: f2
Molecule details ›
Chain: f2
Length: 133 amino acids
Theoretical weight: 14.5 KDa
Source organism: Oryctolagus cuniculus
UniProt:
  • Canonical: G1T8A2 (Residues: 1-133; Coverage: 100%)
Gene name: FAU
Sequence domains:
Small ribosomal subunit protein eS31 Chain: g2
Molecule details ›
Chain: g2
Length: 68 amino acids
Theoretical weight: 7.99 KDa
Source organism: Oryctolagus cuniculus
UniProt:
  • Canonical: G1SK22 (Residues: 83-150; Coverage: 44%)
Gene name: RPS27A
Sequence domains:
Small ribosomal subunit protein RACK1 Chain: h2
Molecule details ›
Chain: h2
Length: 313 amino acids
Theoretical weight: 34.67 KDa
Source organism: Oryctolagus cuniculus
UniProt:
  • Canonical: G1SJB4 (Residues: 2-314; Coverage: 99%)
Gene name: RACK1
Sequence domains: WD domain, G-beta repeat
L8 Chain: A3
Molecule details ›
Chain: A3
Length: 246 amino acids
Theoretical weight: 26.84 KDa
Source organism: Oryctolagus cuniculus
Large ribosomal subunit protein uL3 Chain: B3
Molecule details ›
Chain: B3
Length: 403 amino acids
Theoretical weight: 46.11 KDa
Source organism: Oryctolagus cuniculus
UniProt:
  • Canonical: G1TL06 (Residues: 1-403; Coverage: 100%)
Gene name: RPL3
Sequence domains: Ribosomal protein L3
Large ribosomal subunit protein uL4 Chain: C3
Molecule details ›
Chain: C3
Length: 425 amino acids
Theoretical weight: 47.73 KDa
Source organism: Oryctolagus cuniculus
UniProt:
  • Canonical: G1SVW5 (Residues: 1-378; Coverage: 92%)
Gene name: RPL4
Sequence domains:
Large ribosomal subunit protein uL18 Chain: D3
Molecule details ›
Chain: D3
Length: 297 amino acids
Theoretical weight: 34.48 KDa
Source organism: Oryctolagus cuniculus
UniProt:
  • Canonical: P19949 (Residues: 1-297; Coverage: 100%)
Gene name: RPL5
Large ribosomal subunit protein eL6 Chain: E3
Molecule details ›
Chain: E3
Length: 291 amino acids
Theoretical weight: 33.03 KDa
Source organism: Oryctolagus cuniculus
UniProt:
  • Canonical: G1SKF7 (Residues: 1-291; Coverage: 100%)
Gene name: RPL6
Sequence domains:
Large ribosomal subunit protein uL30 Chain: F3
Molecule details ›
Chain: F3
Length: 249 amino acids
Theoretical weight: 29.51 KDa
Source organism: Oryctolagus cuniculus
UniProt:
  • Canonical: G1SV32 (Residues: 1-208; Coverage: 100%)
Gene name: RPL7
Sequence domains:
Large ribosomal subunit protein eL8 Chain: G3
Molecule details ›
Chain: G3
Length: 241 amino acids
Theoretical weight: 27.48 KDa
Source organism: Oryctolagus cuniculus
UniProt:
  • Canonical: G1STW0 (Residues: 79-319; Coverage: 76%)
Gene name: RPL7A
Sequence domains: Ribosomal protein L7Ae/L30e/S12e/Gadd45 family
Large ribosomal subunit protein uL6 Chain: H3
Molecule details ›
Chain: H3
Length: 190 amino acids
Theoretical weight: 21.63 KDa
Source organism: Oryctolagus cuniculus
UniProt:
  • Canonical: G1SWI6 (Residues: 1-190; Coverage: 99%)
Gene name: RPL9
Sequence domains: Ribosomal protein L6
Large ribosomal subunit protein uL16 Chain: I3
Molecule details ›
Chain: I3
Length: 214 amino acids
Theoretical weight: 24.64 KDa
Source organism: Oryctolagus cuniculus
UniProt:
  • Canonical: B7NZQ2 (Residues: 1-214; Coverage: 100%)
Gene name: RPL10
Sequence domains: Ribosomal protein L16p/L10e
Large ribosomal subunit protein uL5 Chain: J3
Molecule details ›
Chain: J3
Length: 181 amino acids
Theoretical weight: 20.67 KDa
Source organism: Oryctolagus cuniculus
UniProt:
  • Canonical: G1TUB8 (Residues: 3-178; Coverage: 99%)
Gene name: RPL11
Sequence domains:
Large ribosomal subunit protein eL13 Chain: L3
Molecule details ›
Chain: L3
Length: 211 amino acids
Theoretical weight: 24.33 KDa
Source organism: Oryctolagus cuniculus
UniProt:
  • Canonical: G1TKB3 (Residues: 1-211; Coverage: 100%)
Gene name: RPL13
Sequence domains: Ribosomal protein L13e
Large ribosomal subunit protein eL14 Chain: M3
Molecule details ›
Chain: M3
Length: 218 amino acids
Theoretical weight: 23.87 KDa
Source organism: Oryctolagus cuniculus
UniProt:
  • Canonical: G1SZ12 (Residues: 1-218; Coverage: 100%)
Gene name: RPL14
Sequence domains: Ribosomal protein L14
Large ribosomal subunit protein eL15 Chain: N3
Molecule details ›
Chain: N3
Length: 203 amino acids
Theoretical weight: 24.08 KDa
Source organism: Oryctolagus cuniculus
UniProt:
  • Canonical: G1T0C1 (Residues: 2-204; Coverage: 100%)
Gene name: RPL15
Sequence domains: Ribosomal L15
60S RIBOSOMAL PROTEIN UL13 Chain: O3
Molecule details ›
Chain: O3
Length: 203 amino acids
Theoretical weight: 23.53 KDa
Source organism: Oryctolagus cuniculus
uL22 Chain: P3
Molecule details ›
Chain: P3
Length: 199 amino acids
Theoretical weight: 23.06 KDa
Source organism: Oryctolagus cuniculus
L18 Chain: Q3
Molecule details ›
Chain: Q3
Length: 188 amino acids
Theoretical weight: 21.7 KDa
Source organism: Oryctolagus cuniculus
Large ribosomal subunit protein eL19 Chain: R3
Molecule details ›
Chain: R3
Length: 196 amino acids
Theoretical weight: 23.54 KDa
Source organism: Oryctolagus cuniculus
UniProt:
  • Canonical: Q3T0W9 (Residues: 1-196; Coverage: 100%)
Gene name: RPL19
Sequence domains: Ribosomal protein L19e
Large ribosomal subunit protein eL20 Chain: S3
Molecule details ›
Chain: S3
Length: 176 amino acids
Theoretical weight: 20.83 KDa
Source organism: Oryctolagus cuniculus
UniProt:
  • Canonical: A0A287APR1 (Residues: 49-224; Coverage: 79%)
Gene name: RPL18A
Sequence domains: Ribosomal proteins 50S-L18Ae/60S-L20/60S-L18A
Large ribosomal subunit protein eL21 Chain: T3
Molecule details ›
Chain: T3
Length: 159 amino acids
Theoretical weight: 18.48 KDa
Source organism: Oryctolagus cuniculus
UniProt:
  • Canonical: G1SHQ2 (Residues: 2-160; Coverage: 99%)
Gene name: RPL21
Sequence domains: Ribosomal protein L21e
L22 Chain: U3
Molecule details ›
Chain: U3
Length: 128 amino acids
Theoretical weight: 14.8 KDa
Source organism: Oryctolagus cuniculus
Large ribosomal subunit protein uL14 Chain: V3
Molecule details ›
Chain: V3
Length: 131 amino acids
Theoretical weight: 13.97 KDa
Source organism: Oryctolagus cuniculus
UniProt:
  • Canonical: G1T6D1 (Residues: 10-140; Coverage: 94%)
Gene name: RPL23
Sequence domains: Ribosomal protein L14p/L23e
Large ribosomal subunit protein uL23 Chain: X3
Molecule details ›
Chain: X3
Length: 156 amino acids
Theoretical weight: 17.77 KDa
Source organism: Oryctolagus cuniculus
UniProt:
  • Canonical: G1SE76 (Residues: 1-156; Coverage: 100%)
Gene name: RPL23A
Sequence domains:
Large ribosomal subunit protein uL24 Chain: Y3
Molecule details ›
Chain: Y3
Length: 132 amino acids
Theoretical weight: 15.71 KDa
Source organism: Oryctolagus cuniculus
UniProt:
  • Canonical: G1SQH0 (Residues: 1-132; Coverage: 91%)
Gene name: RPL26
Sequence domains:
Large ribosomal subunit protein eL27 Chain: Z3
Molecule details ›
Chain: Z3
Length: 135 amino acids
Theoretical weight: 15.7 KDa
Source organism: Oryctolagus cuniculus
UniProt:
  • Canonical: G1TXF6 (Residues: 2-136; Coverage: 99%)
Gene name: RPL27
Sequence domains:
uL15 Chain: a3
Molecule details ›
Chain: a3
Length: 160 amino acids
Theoretical weight: 17.83 KDa
Source organism: Oryctolagus cuniculus
Large ribosomal subunit protein eL29 Chain: b3
Molecule details ›
Chain: b3
Length: 245 amino acids
Theoretical weight: 26.71 KDa
Source organism: Oryctolagus cuniculus
UniProt:
  • Canonical: G1SGR6 (Residues: 1-226; Coverage: 92%)
Gene name: RPL29
Sequence domains: Ribosomal L29e protein family
Large ribosomal subunit protein eL30 Chain: c3
Molecule details ›
Chain: c3
Length: 115 amino acids
Theoretical weight: 12.81 KDa
Source organism: Oryctolagus cuniculus
UniProt:
  • Canonical: G1TDL2 (Residues: 1-115; Coverage: 100%)
Gene name: RPL30
Sequence domains: Ribosomal protein L7Ae/L30e/S12e/Gadd45 family
Large ribosomal subunit protein eL31 Chain: d3
Molecule details ›
Chain: d3
Length: 107 amino acids
Theoretical weight: 12.64 KDa
Source organism: Oryctolagus cuniculus
UniProt:
  • Canonical: G1SHG0 (Residues: 18-124; Coverage: 86%)
Gene name: RPL31
Sequence domains: Ribosomal protein L31e
Large ribosomal subunit protein eL32 Chain: e3
Molecule details ›
Chain: e3
Length: 128 amino acids
Theoretical weight: 15.02 KDa
Source organism: Oryctolagus cuniculus
UniProt:
  • Canonical: G1TUN8 (Residues: 2-129; Coverage: 95%)
Gene name: RPL32
Sequence domains: Ribosomal protein L32
Large ribosomal subunit protein eL33 Chain: f3
Molecule details ›
Chain: f3
Length: 109 amino acids
Theoretical weight: 12.45 KDa
Source organism: Oryctolagus cuniculus
UniProt:
  • Canonical: G1SF08 (Residues: 2-110; Coverage: 99%)
Gene name: RPL35A
Sequence domains: Ribosomal protein L35Ae
Large ribosomal subunit protein eL34 Chain: g3
Molecule details ›
Chain: g3
Length: 126 amino acids
Theoretical weight: 14.21 KDa
Source organism: Oryctolagus cuniculus
UniProt:
  • Canonical: G1TXG5 (Residues: 1-116; Coverage: 99%)
Gene name: RPL34
Sequence domains: Ribosomal protein L34e
Large ribosomal subunit protein uL29 Chain: h3
Molecule details ›
Chain: h3
Length: 122 amino acids
Theoretical weight: 14.44 KDa
Source organism: Oryctolagus cuniculus
UniProt:
  • Canonical: G1SIT5 (Residues: 2-123; Coverage: 99%)
Gene name: RPL35
Sequence domains: Ribosomal L29 protein
60S ribosomal protein L36 Chain: i3
Molecule details ›
Chain: i3
Length: 117 amino acids
Theoretical weight: 13.55 KDa
Source organism: Oryctolagus cuniculus
Large ribosomal subunit protein eL37 Chain: j3
Molecule details ›
Chain: j3
Length: 97 amino acids
Theoretical weight: 11.11 KDa
Source organism: Oryctolagus cuniculus
UniProt:
  • Canonical: U3KPD5 (Residues: 1-97; Coverage: 100%)
Gene name: RPL37
Sequence domains: Ribosomal protein L37e
L38 Chain: k3
Molecule details ›
Chain: k3
Length: 69 amino acids
Theoretical weight: 8.11 KDa
Source organism: Oryctolagus cuniculus
Large ribosomal subunit protein eL39 Chain: l3
Molecule details ›
Chain: l3
Length: 50 amino acids
Theoretical weight: 6.32 KDa
Source organism: Oryctolagus cuniculus
UniProt:
  • Canonical: G1TTN1 (Residues: 2-51; Coverage: 98%)
Sequence domains: Ribosomal L39 protein
60S RIBOSOMAL PROTEIN EL40 Chain: m3
Molecule details ›
Chain: m3
Length: 128 amino acids
Theoretical weight: 14.7 KDa
Source organism: Oryctolagus cuniculus
Small ribosomal subunit protein eS32 Chain: n3
Molecule details ›
Chain: n3
Length: 25 amino acids
Theoretical weight: 3.47 KDa
Source organism: Oryctolagus cuniculus
UniProt:
  • Canonical: A0A087WNH4 (Residues: 1-25; Coverage: 100%)
Gene name: RPL41
Sequence domains: Ribosomal protein L41
eL42 Chain: o3
Molecule details ›
Chain: o3
Length: 142 amino acids
Theoretical weight: 16.17 KDa
Source organism: Oryctolagus cuniculus
ribosomal protein eL43 Chain: p3
Molecule details ›
Chain: p3
Length: 109 amino acids
Theoretical weight: 12.01 KDa
Source organism: Oryctolagus cuniculus
Large ribosomal subunit protein eL28 Chain: r3
Molecule details ›
Chain: r3
Length: 137 amino acids
Theoretical weight: 15.78 KDa
Source organism: Oryctolagus cuniculus
UniProt:
  • Canonical: G1U7L1 (Residues: 1-137; Coverage: 100%)
Gene name: RPL28
Sequence domains: Ribosomal L28e protein family
Large ribosomal subunit protein uL10 Chain: t3
Molecule details ›
Chain: t3
Length: 318 amino acids
Theoretical weight: 34.38 KDa
Source organism: Oryctolagus cuniculus
UniProt:
  • Canonical: G1SPK4 (Residues: 1-318; Coverage: 100%)
Gene name: RPLP0
Sequence domains:
uL12 Chain: u3
Molecule details ›
Chain: u3
Length: 195 amino acids
Theoretical weight: 21.3 KDa
Source organism: Oryctolagus cuniculus
Large ribosomal subunit protein uL1 Chain: w3
Molecule details ›
Chain: w3
Length: 217 amino acids
Theoretical weight: 24.88 KDa
Source organism: Oryctolagus cuniculus
UniProt:
  • Canonical: G1SKZ8 (Residues: 1-217; Coverage: 100%)
Gene name: RPL10A
Sequence domains: Ribosomal protein L1p/L10e family
Sec61Beta Chain: 1
Molecule details ›
Chain: 1
Length: 15 amino acids
Theoretical weight: 1.79 KDa
Source organism: Oryctolagus cuniculus
Large ribosomal subunit protein eL24 Chain: W
Molecule details ›
Chain: W
Length: 134 amino acids
Theoretical weight: 15.54 KDa
Source organism: Oryctolagus cuniculus
UniProt:
  • Canonical: A0A061I3X8 (Residues: 1-134; Coverage: 100%)
Gene name: H671_4g12624
Sequence domains: Ribosomal protein L24e
28S ribosomal RNA Chain: 51
Molecule details ›
Chain: 51
Length: 3788 nucleotides
Theoretical weight: 1226.62 KDa
Sequence domains: Eukaryotic large subunit ribosomal RNA
5S ribosomal RNA Chain: 71
Molecule details ›
Chain: 71
Length: 120 nucleotides
Theoretical weight: 38.69 KDa
Sequence domains: 5S ribosomal RNA
5.8S ribosomal RNA Chain: 81
Molecule details ›
Chain: 81
Length: 151 nucleotides
Theoretical weight: 48.55 KDa
Sequence domains: 5.8S ribosomal RNA
18S Ribosomal RNA Chain: A2
Molecule details ›
Chain: A2
Length: 1818 nucleotides
Theoretical weight: 586.37 KDa
Sequence domains: Eukaryotic small subunit ribosomal RNA
A/P-Site tRNA Chain: q3
Molecule details ›
Chain: q3
Length: 74 nucleotides
Theoretical weight: 23.83 KDa
Sequence domains: tRNA
Messenger RNA Chain: v3
Molecule details ›
Chain: v3
Length: 18 nucleotides
Theoretical weight: 5.72 KDa
P/E-Site tRNA Chain: 33
Molecule details ›
Chain: 33
Length: 75 nucleotides
Theoretical weight: 24.12 KDa
Sequence domains: tRNA

Ligands and Environments

3 bound ligands:
Ligand MG 299 x MG
Ligand BLS 1 x BLS
Ligand ZN 8 x ZN
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 3.8Å
Relevant EMDB volumes: EMD-12631

Quick links

Citations

2 review citations

Inhibition of the Eukaryotic 80S Ribosome as a Potential Anticancer Therapy: A Structural Perspective.
Pellegrino et al. (2021)
1 more

2 mentions without citation

Blasticidin S inhibits mammalian translation and enhances production of protein encoded by nonsense mRNA.
Powers et al. (2021)
1 more