› 7nwr

X-ray diffraction
2Å resolution

Structure of BT1526, a myo-inositol-1-phosphate synthase

Released:
DOI: 10.2210/pdb7nwr/pdb
PDB entry 7nwr coloured by chain, front view.
PDB entry 7nwr coloured by chain, side view.
PDB entry 7nwr coloured by chain, top view.
Source organism: Bacteroides thetaiotaomicron
Primary publication:
Characterization of inositol lipid metabolism in gut-associated Bacteroidetes.
Heaver SL, Le HH, Tang P, Baslé A, Mirretta Barone C, Vu DL, Waters JL, Marles-Wright J, Johnson EL, Campopiano DJ, Ley RE
Nat Microbiol 7 986-1000 (2022)
PMID: 35725777

Function and Biology Details

Reaction catalysed: 
D-glucose 6-phosphate = 1D-myo-inositol 3-phosphate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-183738 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Inositol-3-phosphate synthase 1 Chains: A, B
Molecule details ›
Chains: A, B
Length: 449 amino acids
Theoretical weight: 50.63 KDa
Source organism: Bacteroides thetaiotaomicron
Expression system: Escherichia coli
UniProt:
  • Canonical: Q8A7J8 (Residues: 1-429; Coverage: 100%)
Gene name: BT_1526
Sequence domains:

Ligands and Environments


Cofactor: Ligand NAD 2 x NAD
1 bound ligand:
Ligand NA 1 x NA
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: DIAMOND BEAMLINE I04
Unit cell:
a: 66.805Å b: 104.563Å c: 124.318Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.185 0.183 0.225
Expression system: Escherichia coli

Quick links

Citations

2 review citations

Gut microbiome lipid metabolism and its impact on host physiology.
Brown et al. (2023)
1 more