› 7o2n

X-ray diffraction
2.8Å resolution

Crystal structure of B. subtilis UGPase YngB

Released:
DOI: 10.2210/pdb7o2n/pdb
PDB entry 7o2n coloured by chain, front view.
PDB entry 7o2n coloured by chain, side view.
PDB entry 7o2n coloured by chain, top view.
Source organism: Bacillus subtilis subsp. subtilis str. 168
Primary publication:
Structural and Functional Characterisation of the Bacillus subtilis Uridylyltransferase YngB
Wu C
Ph.D.Thesis (2021)

Function and Biology Details

Reaction catalysed: 
UTP + alpha-D-glucose 1-phosphate = diphosphate + UDP-glucose
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-128381 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
UTP--glucose-1-phosphate uridylyltransferase YngB Chains: A, B
Molecule details ›
Chains: A, B
Length: 296 amino acids
Theoretical weight: 33.06 KDa
Source organism: Bacillus subtilis subsp. subtilis str. 168
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: O31822 (Residues: 2-297; Coverage: 100%)
Gene names: BSU18180, yngB
Sequence domains: Nucleotidyl transferase

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: DIAMOND BEAMLINE I04
Spacegroup: I212121
Unit cell:
a: 54.156Å b: 158.979Å c: 180.317Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.216 0.213 0.264
Expression system: Escherichia coli BL21(DE3)

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