› 7o3e

Electron Microscopy
3.6Å resolution

Murine supercomplex CIII2CIV in the intermediate locked conformation

Released:
DOI: 10.2210/pdb7o3e/pdb
PDB entry 7o3e coloured by chain, front view.
PDB entry 7o3e coloured by chain, side view.
PDB entry 7o3e coloured by chain, top view.
Source organism: Mus musculus
Primary publication:
Structure and assembly of the mammalian mitochondrial supercomplex CIII2CIV.
Vercellino I, Sazanov LA
Nature 598 364-367 (2021)
PMID: 34616041
Related structures: EMD-12705

Function and Biology Details

Reactions catalysed: 
Quinol + 2 ferricytochrome c(Side 2) = quinone + 2 ferrocytochrome c(Side 2) + 2 H(+)(Side 2)
4 ferrocytochrome c + O(2) + 8 H(+)(Side 1) = 4 ferricytochrome c + 4 H(2)O(Side 2)
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero 31-mer (preferred)
PDBe Complex ID:
PDB-CPX-132298 (preferred)
Entry contents:
23 distinct polypeptide molecules
Macromolecules (23 distinct):
Cytochrome b-c1 complex subunit 1, mitochondrial Chains: A, L
Molecule details ›
Chains: A, L
Length: 446 amino acids
Theoretical weight: 49.36 KDa
Source organism: Mus musculus
UniProt:
  • Canonical: Q9CZ13 (Residues: 35-480; Coverage: 93%)
Gene name: Uqcrc1
Sequence domains:
Cytochrome b-c1 complex subunit 2, mitochondrial Chains: B, M
Molecule details ›
Chains: B, M
Length: 439 amino acids
Theoretical weight: 46.64 KDa
Source organism: Mus musculus
UniProt:
  • Canonical: Q9DB77 (Residues: 15-453; Coverage: 97%)
Gene name: Uqcrc2
Sequence domains:
Cytochrome b Chains: C, N
Molecule details ›
Chains: C, N
Length: 381 amino acids
Theoretical weight: 43.24 KDa
Source organism: Mus musculus
UniProt:
  • Canonical: P00158 (Residues: 1-381; Coverage: 100%)
Gene names: Cob, Cytb, Mt-Cyb, Mtcyb, mt-Cytb
Sequence domains:
Cytochrome c1, heme protein, mitochondrial Chains: D, O
Molecule details ›
Chains: D, O
Length: 241 amino acids
Theoretical weight: 27.31 KDa
Source organism: Mus musculus
UniProt:
  • Canonical: Q9D0M3 (Residues: 85-325; Coverage: 74%)
Gene name: Cyc1
Sequence domains: Cytochrome C1 family
Cytochrome b-c1 complex subunit 7 Chains: F, Q
Molecule details ›
Chains: F, Q
Length: 110 amino acids
Theoretical weight: 13.46 KDa
Source organism: Mus musculus
UniProt:
  • Canonical: Q9D855 (Residues: 2-111; Coverage: 99%)
Gene name: Uqcrb
Sequence domains: Ubiquinol-cytochrome C reductase complex 14kD subunit
Cytochrome b-c1 complex subunit 8 Chains: G, R
Molecule details ›
Chains: G, R
Length: 81 amino acids
Theoretical weight: 9.65 KDa
Source organism: Mus musculus
UniProt:
  • Canonical: Q9CQ69 (Residues: 2-82; Coverage: 99%)
Gene name: Uqcrq
Sequence domains: UcrQ family
Cytochrome b-c1 complex subunit 6, mitochondrial Chains: H, S
Molecule details ›
Chains: H, S
Length: 76 amino acids
Theoretical weight: 9 KDa
Source organism: Mus musculus
UniProt:
  • Canonical: P99028 (Residues: 14-89; Coverage: 85%)
Gene name: Uqcrh
Sequence domains: Ubiquinol-cytochrome C reductase hinge protein
Cytochrome b-c1 complex subunit Rieske, mitochondrial Chain: P
Molecule details ›
Chain: P
Length: 196 amino acids
Theoretical weight: 21.52 KDa
Source organism: Mus musculus
UniProt:
  • Canonical: Q9CR68 (Residues: 79-274; Coverage: 72%)
Gene name: Uqcrfs1
Sequence domains:
Cytochrome b-c1 complex subunit 9 Chain: T
Molecule details ›
Chain: T
Length: 78 amino acids
Theoretical weight: 7.9 KDa
Source organism: Mus musculus
UniProt:
  • Canonical: Q9CR68 (Residues: 1-78; Coverage: 29%)
Gene name: Uqcrfs1
Sequence domains: Ubiquinol-cytochrome c reductase 8 kDa, N-terminal
Cox7a2l protein Chain: I
Molecule details ›
Chain: I
Length: 113 amino acids
Theoretical weight: 12.58 KDa
Source organism: Mus musculus
UniProt:
  • Canonical: Q99KD6 (Residues: 1-113; Coverage: 100%)
Gene names: Cox7a2l, Gm6969
Sequence domains: Cytochrome c oxidase subunit VII
Cytochrome c oxidase subunit 1 Chain: a
Molecule details ›
Chain: a
Length: 514 amino acids
Theoretical weight: 56.95 KDa
Source organism: Mus musculus
UniProt:
  • Canonical: P00397 (Residues: 1-514; Coverage: 100%)
Gene names: COI, Mtco1, mt-Co1
Sequence domains: Cytochrome C and Quinol oxidase polypeptide I
Cytochrome c oxidase subunit 2 Chain: b
Molecule details ›
Chain: b
Length: 227 amino acids
Theoretical weight: 25.99 KDa
Source organism: Mus musculus
UniProt:
  • Canonical: P00405 (Residues: 1-227; Coverage: 100%)
Gene names: COII, COX2, Mtco2, mt-Co2
Sequence domains:
Cytochrome c oxidase subunit 3 Chain: c
Molecule details ›
Chain: c
Length: 261 amino acids
Theoretical weight: 29.95 KDa
Source organism: Mus musculus
UniProt:
  • Canonical: P00416 (Residues: 1-261; Coverage: 100%)
Gene names: COIII, Mtco3, mt-Co3
Sequence domains: Cytochrome c oxidase subunit III
Cytochrome c oxidase subunit 4 isoform 1, mitochondrial Chain: d
Molecule details ›
Chain: d
Length: 147 amino acids
Theoretical weight: 17.23 KDa
Source organism: Mus musculus
UniProt:
  • Canonical: P19783 (Residues: 23-169; Coverage: 87%)
Gene names: Cox4, Cox4a, Cox4i1, Coxiv
Sequence domains: Cytochrome c oxidase subunit IV
Cytochrome c oxidase subunit 5A, mitochondrial Chain: e
Molecule details ›
Chain: e
Length: 109 amino acids
Theoretical weight: 12.45 KDa
Source organism: Mus musculus
UniProt:
  • Canonical: P12787 (Residues: 38-146; Coverage: 75%)
Gene name: Cox5a
Sequence domains: Cytochrome c oxidase subunit Va
Cytochrome c oxidase subunit 5B, mitochondrial Chain: f
Molecule details ›
Chain: f
Length: 99 amino acids
Theoretical weight: 10.86 KDa
Source organism: Mus musculus
UniProt:
  • Canonical: P19536 (Residues: 30-128; Coverage: 77%)
Gene name: Cox5b
Sequence domains: Cytochrome c oxidase subunit Vb
Cytochrome c oxidase subunit 6A2, mitochondrial Chain: g
Molecule details ›
Chain: g
Length: 85 amino acids
Theoretical weight: 9.44 KDa
Source organism: Mus musculus
UniProt:
  • Canonical: P43023 (Residues: 13-97; Coverage: 88%)
Gene name: Cox6a2
Sequence domains: Cytochrome c oxidase subunit VIa
Cytochrome c oxidase subunit 6B1 Chain: h
Molecule details ›
Chain: h
Length: 85 amino acids
Theoretical weight: 9.96 KDa
Source organism: Mus musculus
UniProt:
  • Canonical: P56391 (Residues: 2-86; Coverage: 99%)
Gene names: Cox6b, Cox6b1
Sequence domains: Cytochrome oxidase c subunit VIb
Cytochrome c oxidase subunit 6C Chain: i
Molecule details ›
Chain: i
Length: 75 amino acids
Theoretical weight: 8.35 KDa
Source organism: Mus musculus
UniProt:
  • Canonical: Q9CPQ1 (Residues: 2-76; Coverage: 99%)
Gene name: Cox6c
Sequence domains: Cytochrome c oxidase subunit VIc
Cytochrome c oxidase subunit 7B, mitochondrial Chain: k
Molecule details ›
Chain: k
Length: 56 amino acids
Theoretical weight: 6.32 KDa
Source organism: Mus musculus
UniProt:
  • Canonical: P56393 (Residues: 25-80; Coverage: 70%)
Gene name: Cox7b
Sequence domains: Cytochrome C oxidase chain VIIB
Cytochrome c oxidase subunit 7C, mitochondrial Chain: l
Molecule details ›
Chain: l
Length: 47 amino acids
Theoretical weight: 5.45 KDa
Source organism: Mus musculus
UniProt:
  • Canonical: P17665 (Residues: 17-63; Coverage: 75%)
Gene names: Cox7c, Cox7c1
Sequence domains: Cytochrome c oxidase subunit VIIc
Cytochrome c oxidase subunit 8B, mitochondrial Chain: m
Molecule details ›
Chain: m
Length: 46 amino acids
Theoretical weight: 4.66 KDa
Source organism: Mus musculus
UniProt:
  • Canonical: P48772 (Residues: 25-70; Coverage: 66%)
Gene names: Cox8b, Cox8h
Sequence domains: Cytochrome oxidase c subunit VIII
Cytochrome b-c1 complex subunit 9 Chains: J, U
Molecule details ›
Chains: J, U
Length: 63 amino acids
Theoretical weight: 7.33 KDa
Source organism: Mus musculus
UniProt:
  • Canonical: Q8R1I1 (Residues: 2-64; Coverage: 98%)
Gene name: Uqcr10
Sequence domains: Ubiquinol-cytochrome C reductase, UQCRX/QCR9 like

Ligands and Environments

12 bound ligands:
Ligand HEM 4 x HEM
Ligand 3PE 13 x 3PE
Ligand CDL 5 x CDL
Ligand HEC 2 x HEC
Ligand PC1 2 x PC1
Ligand CU 1 x CU
Ligand NA 1 x NA
Ligand HEA 2 x HEA
Ligand MG 1 x MG
Ligand CUA 1 x CUA
Ligand ZN 1 x ZN
Ligand TGL 1 x TGL
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 3.6Å
Relevant EMDB volumes: EMD-12705

Quick links

Citations

10 review citations

Metabolic regulation of endothelial senescence.
Le NT. (2023)
9 more