› 7o3e

Electron Microscopy
3.6Å resolution

Murine supercomplex CIII2CIV in the intermediate locked conformation

Released:
DOI: 10.2210/pdb7o3e/pdb
PDB entry 7o3e coloured by chain, front view.
PDB entry 7o3e coloured by chain, side view.
PDB entry 7o3e coloured by chain, top view.
Source organism: Mus musculus
Primary publication:
Structure and assembly of the mammalian mitochondrial supercomplex CIII2CIV.
Vercellino I, Sazanov LA
Nature 598 364-367 (2021)
PMID: 34616041
Related structures: EMD-12705

Function and Biology Details

Reactions catalysed: 
Quinol + 2 ferricytochrome c(Side 2) = quinone + 2 ferrocytochrome c(Side 2) + 2 H(+)(Side 2)
4 ferrocytochrome c + O(2) + 8 H(+)(Side 1) = 4 ferricytochrome c + 4 H(2)O(Side 2)
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero 31-mer (preferred)
PDBe Complex ID:
PDB-CPX-279394 (preferred)
Entry contents:
23 distinct polypeptide molecules
Macromolecules (23 distinct):
Cytochrome b-c1 complex subunit 1, mitochondrial Chains: A, L
Molecule details ›
Chains: A, L
Length: 446 amino acids
Theoretical weight: 49.36 KDa
Source organism: Mus musculus
UniProt:
  • Canonical: Q9CZ13 (Residues: 35-480; Coverage: 93%)
Gene name: Uqcrc1
Sequence domains:
Cytochrome b-c1 complex subunit 2, mitochondrial Chains: B, M
Molecule details ›
Chains: B, M
Length: 439 amino acids
Theoretical weight: 46.64 KDa
Source organism: Mus musculus
UniProt:
  • Canonical: Q9DB77 (Residues: 15-453; Coverage: 97%)
Gene name: Uqcrc2
Sequence domains:
Cytochrome b Chains: C, N
Cytochrome c1, heme protein, mitochondrial Chains: D, O
Cytochrome b-c1 complex subunit 7 Chains: F, Q
Cytochrome b-c1 complex subunit 8 Chains: G, R
Cytochrome b-c1 complex subunit 6, mitochondrial Chains: H, S
Cytochrome b-c1 complex subunit Rieske, mitochondrial Chain: P
Cytochrome b-c1 complex subunit 9 Chain: T
Cytochrome c oxidase subunit 7A2-like, mitochondrial Chain: I
Cytochrome c oxidase subunit 1 Chain: a
Cytochrome c oxidase subunit 2 Chain: b
Cytochrome c oxidase subunit 3 Chain: c
Cytochrome c oxidase subunit 4 isoform 1, mitochondrial Chain: d
Cytochrome c oxidase subunit 5A, mitochondrial Chain: e
Cytochrome c oxidase subunit 5B, mitochondrial Chain: f
Cytochrome c oxidase subunit 6A2, mitochondrial Chain: g
Cytochrome c oxidase subunit 6B1 Chain: h
Cytochrome c oxidase subunit 6C Chain: i
Cytochrome c oxidase subunit 7B, mitochondrial Chain: k
Cytochrome c oxidase subunit 7C, mitochondrial Chain: l
Cytochrome c oxidase subunit 8B, mitochondrial Chain: m
Cytochrome b-c1 complex subunit 9 Chains: J, U

Ligands and Environments

12 bound ligands:
Ligand HEM 4 x HEM
Ligand 3PE 13 x 3PE
Ligand CDL 5 x CDL
Ligand HEC 2 x HEC
Ligand PC1 2 x PC1
Ligand CU 1 x CU
Ligand NA 1 x NA
Ligand HEA 2 x HEA
Ligand MG 1 x MG
Ligand CUA 1 x CUA
Ligand ZN 1 x ZN
Ligand TGL 1 x TGL
No modified residues

Experiments and Validation Details

wwPDB Validation report is not available for this EM entry.
Resolution: 3.6Å
Relevant EMDB volumes: EMD-12705

Quick links

Citations

10 review citations

Mechanisms of mitochondrial respiratory adaptation.
Bennett et al. (2022)
9 more