› 7o3h

Electron Microscopy
2.6Å resolution

Murine CIII2 focus-refined from supercomplex CICIII2

Released:
DOI: 10.2210/pdb7o3h/pdb
PDB entry 7o3h coloured by chain, front view.
PDB entry 7o3h coloured by chain, side view.
PDB entry 7o3h coloured by chain, top view.
Source organism: Mus musculus
Primary publication:
Structure and assembly of the mammalian mitochondrial supercomplex CIII2CIV.
Vercellino I, Sazanov LA
Nature 598 364-367 (2021)
PMID: 34616041
Related structures: EMD-12706

Function and Biology Details

Reaction catalysed: 
Quinol + 2 ferricytochrome c(Side 2) = quinone + 2 ferrocytochrome c(Side 2) + 2 H(+)(Side 2)
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero 21-mer (preferred)
PDBe Complex ID:
PDB-CPX-132299 (preferred)
Entry contents:
11 distinct polypeptide molecules
Macromolecules (11 distinct):
Cytochrome b-c1 complex subunit 1, mitochondrial Chains: A, L
Molecule details ›
Chains: A, L
Length: 446 amino acids
Theoretical weight: 49.36 KDa
Source organism: Mus musculus
UniProt:
  • Canonical: Q9CZ13 (Residues: 35-480; Coverage: 93%)
Gene name: Uqcrc1
Sequence domains:
Cytochrome b-c1 complex subunit 2, mitochondrial Chains: B, M
Molecule details ›
Chains: B, M
Length: 439 amino acids
Theoretical weight: 46.64 KDa
Source organism: Mus musculus
UniProt:
  • Canonical: Q9DB77 (Residues: 15-453; Coverage: 97%)
Gene name: Uqcrc2
Sequence domains:
Cytochrome b Chains: C, N
Molecule details ›
Chains: C, N
Length: 381 amino acids
Theoretical weight: 43.24 KDa
Source organism: Mus musculus
UniProt:
  • Canonical: P00158 (Residues: 1-381; Coverage: 100%)
Gene names: Cob, Cytb, Mt-Cyb, Mtcyb, mt-Cytb
Sequence domains:
Cytochrome c1, heme protein, mitochondrial Chains: D, O
Molecule details ›
Chains: D, O
Length: 241 amino acids
Theoretical weight: 27.31 KDa
Source organism: Mus musculus
UniProt:
  • Canonical: Q9D0M3 (Residues: 85-325; Coverage: 74%)
Gene name: Cyc1
Sequence domains: Cytochrome C1 family
Cytochrome b-c1 complex subunit Rieske, mitochondrial Chains: E, P
Molecule details ›
Chains: E, P
Length: 196 amino acids
Theoretical weight: 21.52 KDa
Source organism: Mus musculus
UniProt:
  • Canonical: Q9CR68 (Residues: 79-274; Coverage: 72%)
Gene name: Uqcrfs1
Sequence domains:
Cytochrome b-c1 complex subunit 7 Chains: F, Q
Molecule details ›
Chains: F, Q
Length: 110 amino acids
Theoretical weight: 13.46 KDa
Source organism: Mus musculus
UniProt:
  • Canonical: Q9D855 (Residues: 2-111; Coverage: 99%)
Gene name: Uqcrb
Sequence domains: Ubiquinol-cytochrome C reductase complex 14kD subunit
Cytochrome b-c1 complex subunit 8 Chains: G, R
Molecule details ›
Chains: G, R
Length: 81 amino acids
Theoretical weight: 9.65 KDa
Source organism: Mus musculus
UniProt:
  • Canonical: Q9CQ69 (Residues: 2-82; Coverage: 99%)
Gene name: Uqcrq
Sequence domains: UcrQ family
Cytochrome b-c1 complex subunit 6, mitochondrial Chains: H, S
Molecule details ›
Chains: H, S
Length: 76 amino acids
Theoretical weight: 9 KDa
Source organism: Mus musculus
UniProt:
  • Canonical: P99028 (Residues: 14-89; Coverage: 85%)
Gene name: Uqcrh
Sequence domains: Ubiquinol-cytochrome C reductase hinge protein
Cytochrome b-c1 complex subunit 9 Chains: J, U
Molecule details ›
Chains: J, U
Length: 63 amino acids
Theoretical weight: 7.33 KDa
Source organism: Mus musculus
UniProt:
  • Canonical: Q8R1I1 (Residues: 2-64; Coverage: 98%)
Gene name: Uqcr10
Sequence domains: Ubiquinol-cytochrome C reductase, UQCRX/QCR9 like
Cytochrome b-c1 complex subunit 10 Chains: K, V
Molecule details ›
Chains: K, V
Length: 56 amino acids
Theoretical weight: 6.55 KDa
Source organism: Mus musculus
UniProt:
  • Canonical: Q9CPX8 (Residues: 1-56; Coverage: 100%)
Gene names: Uqcr, Uqcr11
Sequence domains: Ubiquinol-cytochrome C reductase complex, 6.4kD protein
Cytochrome b-c1 complex subunit 9 Chain: T
Molecule details ›
Chain: T
Length: 78 amino acids
Theoretical weight: 7.9 KDa
Source organism: Mus musculus
UniProt:
  • Canonical: Q9CR68 (Residues: 1-78; Coverage: 29%)
Gene name: Uqcrfs1
Sequence domains: Ubiquinol-cytochrome c reductase 8 kDa, N-terminal

Ligands and Environments

6 bound ligands:
Ligand 3PE 8 x 3PE
Ligand CDL 6 x CDL
Ligand HEM 4 x HEM
Ligand HEC 2 x HEC
Ligand FES 2 x FES
Ligand PC1 2 x PC1
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 2.6Å
Relevant EMDB volumes: EMD-12706

Quick links

Citations

10 review citations

Metabolic regulation of endothelial senescence.
Le NT. (2023)
9 more

1 mention without citation

Structural basis of mitochondrial membrane bending by the I-II-III2-IV2 supercomplex.
Mühleip et al. (2023)