› 7o4b

X-ray diffraction
2.59Å resolution

Crystal structure of Penicillin-Binding Protein 1 (PBP1) from Staphylococcus aureus in complex with penicillin G

Released:
DOI: 10.2210/pdb7o4b/pdb
PDB entry 7o4b coloured by chain, front view.
PDB entry 7o4b coloured by chain, side view.
PDB entry 7o4b coloured by chain, top view.
Source organism: Staphylococcus aureus subsp. aureus COL
Primary publication:
Integrative structural biology of the penicillin-binding protein-1 from Staphylococcus aureus, an essential component of the divisome machinery.
Martínez-Caballero S, Mahasenan KV, Kim C, Molina R, Feltzer R, Lee M, Bouley R, Hesek D, Fisher JF, Muñoz IG, Chang M, Mobashery S, Hermoso JA
Comput Struct Biotechnol J 19 5392-5405 (2021)
PMID: 34667534

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-101601 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
PASTA domain-containing protein Chains: A, C
Molecule details ›
Chains: A, C
Length: 650 amino acids
Theoretical weight: 72.36 KDa
Source organism: Staphylococcus aureus subsp. aureus COL
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: A0A0H2WVW5 (Residues: 65-713; Coverage: 87%)
Gene names: SACOL1194, pbp1
Sequence domains:

Ligands and Environments

3 bound ligands:
Ligand CIT 3 x CIT
Ligand SO4 11 x SO4
Ligand PNM 2 x PNM
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ALBA BEAMLINE XALOC
Spacegroup: P6422
Unit cell:
a: 180.649Å b: 180.649Å c: 223.466Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.207 0.205 0.242
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

2 citation in other articles

Penicillin-Binding Protein 1 (PBP1) of Staphylococcus aureus Has Multiple Essential Functions in Cell Division.
Wacnik et al. (2022)
1 more

1 mention without citation

Integrative structural biology of the penicillin-binding protein-1 from Staphylococcus aureus, an essential component of the divisome machinery.
Martínez-Caballero et al. (2021)