› 7o8b

X-ray diffraction
1.75Å resolution

Structure of haloalkane dehalogenase variant DhaA80 from Rhodococcus rhodochrous

Released:
DOI: 10.2210/pdb7o8b/pdb
PDB entry 7o8b coloured by chain, front view.
PDB entry 7o8b coloured by chain, side view.
PDB entry 7o8b coloured by chain, top view.
Source organism: Rhodococcus rhodochrous
Primary publication:
Stabilization of Haloalkane Dehalogenase Structure by Interfacial Interaction with Ionic Liquids
Shaposhnikova A, Kuty M, Chaloupkova R, Damborsky J, Kuta Smatanova I, Minofar B, Prudnikova T
Crystals 11 (2021)

Function and Biology Details

Reaction catalysed: 
1-haloalkane + H(2)O = a primary alcohol + halide
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-141208 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Haloalkane dehalogenase Chain: A
Molecule details ›
Chain: A
Length: 290 amino acids
Theoretical weight: 33.09 KDa
Source organism: Rhodococcus rhodochrous
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P0A3G2 (Residues: 4-293; Coverage: 99%)
Gene name: dhaA
Sequence domains: alpha/beta hydrolase fold

Ligands and Environments

1 bound ligand:
Ligand V5B 1 x V5B
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: BESSY BEAMLINE 14.2
Spacegroup: P212121
Unit cell:
a: 50.889Å b: 69.551Å c: 83.916Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.209 0.208 0.224
Expression system: Escherichia coli BL21(DE3)

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