› 7oa5

X-ray diffraction
2.38Å resolution

RUVA COMPLEXED TO A HOLLIDAY JUNCTION.

Released:
DOI: 10.2210/pdb7oa5/pdb
PDB entry 7oa5 coloured by chain, front view.
PDB entry 7oa5 coloured by chain, side view.
PDB entry 7oa5 coloured by chain, top view.
Source organisms:
Primary publication:
Crystal structure of an octameric RuvA-Holliday junction complex
Roe SM, Pearl LH
Molecular Cell 2 361-372 (1998)

Function and Biology Details

Reaction catalysed: 
ATP + H(2)O = ADP + phosphate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero dodecamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-121719 (preferred)
Entry contents:
1 distinct polypeptide molecule
4 distinct DNA molecules
Macromolecules (5 distinct):
Holliday junction branch migration complex subunit RuvA Chains: A, B, C, D, E, F, G, H
Molecule details ›
Chains: A, B, C, D, E, F, G, H
Length: 203 amino acids
Theoretical weight: 20.77 KDa
Source organism: Mycobacterium leprae TN
Expression system: Escherichia coli
UniProt:
  • Canonical: P40832 (Residues: 1-203; Coverage: 100%)
Gene names: B1177_C2_188, ML0482, ruvA
Sequence domains:
DNA (5'-D(*AP*GP*TP*TP*CP*GP*CP*GP*AP*GP*TP*TP*CP*GP*C)-3') Chain: I
Molecule details ›
Chain: I
Length: 15 nucleotides
Theoretical weight: 4.6 KDa
Source organism: Mycobacterium leprae
Expression system: Not provided
DNA (5'-D(*GP*CP*GP*AP*AP*CP*TP*CP*GP*CP*GP*AP*AP*C)-3') Chain: J
Molecule details ›
Chain: J
Length: 14 nucleotides
Theoretical weight: 4.27 KDa
Source organism: Mycobacterium leprae
Expression system: Not provided
DNA (5'-D(*AP*GP*TP*TP*CP*GP*CP*GP*CP*GP*CP*GP*AP*AP*CP*T)-3') Chain: K
Molecule details ›
Chain: K
Length: 16 nucleotides
Theoretical weight: 4.9 KDa
Source organism: Mycobacterium leprae
Expression system: Not provided
DNA (5'-D(P*GP*TP*TP*CP*GP*CP*GP*CP*GP*CP*GP*AP*AP*CP*T)-3') Chain: L
Molecule details ›
Chain: L
Length: 15 nucleotides
Theoretical weight: 4.59 KDa
Source organism: Mycobacterium leprae
Expression system: Not provided

Ligands and Environments

1 bound ligand:
Ligand CA 2 x CA
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SRS BEAMLINE PX7.2
Spacegroup: P31
Unit cell:
a: 141.35Å b: 141.35Å c: 106.47Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.228 0.227 0.25
Expression systems:
  • Escherichia coli
  • Not provided

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