› 7oaj

X-ray diffraction
1.93Å resolution

Crystal structure of pseudokinase CASK in complex with compound 7

Released:
DOI: 10.2210/pdb7oaj/pdb
PDB entry 7oaj coloured by chain, front view.
PDB entry 7oaj coloured by chain, side view.
PDB entry 7oaj coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Design and Development of a Chemical Probe for Pseudokinase Ca2+/calmodulin-Dependent Ser/Thr Kinase.
Russ N, Schröder M, Berger BT, Mandel S, Aydogan Y, Mauer S, Pohl C, Drewry DH, Chaikuad A, Müller S, Knapp S
J Med Chem 64 14358-14376 (2021)
PMID: 34543009

Function and Biology Details

Reaction catalysed: 
ATP + a protein = ADP + a phosphoprotein
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-127208 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Peripheral plasma membrane protein CASK Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 353 amino acids
Theoretical weight: 39.51 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: O14936 (Residues: 1-337; Coverage: 36%)
Gene names: CASK, LIN2
Sequence domains: Protein kinase domain

Ligands and Environments

2 bound ligands:
Ligand V6E 4 x V6E
Ligand EDO 22 x EDO
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SLS BEAMLINE X06SA
Spacegroup: P1
Unit cell:
a: 41.355Å b: 66.844Å c: 130.203Å
α: 76.2° β: 89.99° γ: 90°
R-values:
R R work R free
0.196 0.195 0.224
Expression system: Escherichia coli BL21(DE3)

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Citations

1 citations in other articles

Drosophila CASK regulates brain size and neuronal morphogenesis, providing a genetic model of postnatal microcephaly suitable for drug discovery.
Tello et al. (2023)