› 7ocx

X-ray diffraction
1.7Å resolution

Crystal Structure of the PID-3 TOFU-6 RRM domain complex

Released:
DOI: 10.2210/pdb7ocx/pdb
PDB entry 7ocx coloured by chain, front view.
PDB entry 7ocx coloured by chain, side view.
PDB entry 7ocx coloured by chain, top view.
Source organism: Caenorhabditis elegans
Primary publication:
Structural basis of PETISCO complex assembly during piRNA biogenesis in C. elegans.
Perez-Borrajero C, Podvalnaya N, Holleis K, Lichtenberger R, Karaulanov E, Simon B, Basquin J, Hennig J, Ketting RF, Falk S
Genes Dev 35 1304-1323 (2021)
PMID: 34413138

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-131235 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Protein pid-3 Chains: A, B
Molecule details ›
Chains: A, B
Length: 84 amino acids
Theoretical weight: 9.16 KDa
Source organism: Caenorhabditis elegans
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: O76616 (Residues: 196-274; Coverage: 26%)
Gene names: Y23H5A.3, pics-1, pid-3
Embryonic developmental protein tofu-6 Chains: C, D
Molecule details ›
Chains: C, D
Length: 103 amino acids
Theoretical weight: 11.39 KDa
Source organism: Caenorhabditis elegans
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q09293 (Residues: 1-99; Coverage: 27%)
Gene names: EEED8.1, mel-47, tofu-6
Sequence domains: RNA recognition motif

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SLS BEAMLINE X10SA
Spacegroup: P212121
Unit cell:
a: 43.98Å b: 68.002Å c: 130.585Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.196 0.194 0.232
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

3 review citations

Germ granules and gene regulation in the Caenorhabditis elegans germline.
Phillips et al. (2022)
2 more

1 mention without citation

Structural basis of PETISCO complex assembly during piRNA biogenesis in C. elegans.
Perez-Borrajero et al. (2021)