› 7omr

X-ray diffraction
1.5Å resolution

Crystal structure of coelenteramide-bound Renilla reniformis luciferase RLuc8-D162A variant

Released:
DOI: 10.2210/pdb7omr/pdb
PDB entry 7omr coloured by chain, front view.
PDB entry 7omr coloured by chain, side view.
PDB entry 7omr coloured by chain, top view.
Source organism: Renilla reniformis
Primary publication:
Catalytic mechanism for Renilla-type luciferases
Schenkmayerova A, Toul M, Pluskal D, Baatallah R, Gagnot G, Pinto GP, Santana VT, Stuchla M, Neugebauer P, Chaiyen P, Damborsky J, Bednar D, Janin YL, Prokop Z, Marek M
Nat Catal (2023)

Function and Biology Details

Reaction catalysed: 
(1a) coelenterazine h + O(2) = coelenterazine h dioxetanone
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-151045 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Coelenterazine h 2-monooxygenase Chain: A
Molecule details ›
Chain: A
Length: 317 amino acids
Theoretical weight: 36.9 KDa
Source organism: Renilla reniformis
Expression system: Escherichia coli
UniProt:
  • Canonical: P27652 (Residues: 1-311; Coverage: 100%)
Sequence domains: alpha/beta hydrolase fold

Ligands and Environments

2 bound ligands:
Ligand CEI 1 x CEI
Ligand GOL 4 x GOL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SLS BEAMLINE X06DA
Unit cell:
a: 56.445Å b: 74.166Å c: 80.346Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.171 0.17 0.186
Expression system: Escherichia coli

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