› 7oud

X-ray diffraction
2.3Å resolution

Crystal structure of a ternary complex of the flavoprotein monooxygenase GrhO5 with FAD and collinone

Released:
DOI: 10.2210/pdb7oud/pdb
Model geometry
Fit model/data
PDB entry 7oud coloured by chain, front view.
PDB entry 7oud coloured by chain, side view.
PDB entry 7oud coloured by chain, top view.
Source organism: Streptomyces sp. JP95
Primary publication:
Catalytic Control of Spiroketal Formation in Rubromycin Polyketide Biosynthesis.
Toplak M, Saleem-Batcha R, Piel J, Teufel R
OpenAccess logo Angew Chem Int Ed Engl 60 26960-26970 (2021)
PMID: 34652045

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-185215 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
FAD-binding domain-containing protein Chain: AAA
Molecule details ›
Chain: AAA
Length: 537 amino acids
Theoretical weight: 56.12 KDa
Source organism: Streptomyces sp. JP95
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q8KSX7 (Residues: 1-537; Coverage: 100%)
Gene name: grhO5
Sequence domains:

Ligands and Environments

2 bound ligands:
Ligand FAD 1 x FAD
Ligand 1J0 1 x 1J0
No modified residues

Experiments and Validation Details

wwPDB Validation report is not available for this entry.
X-ray source: SLS BEAMLINE X06SA
Spacegroup: P21
Unit cell:
a: 45.666Å b: 103.747Å c: 69.005Å
α: 90° β: 104.594° γ: 90°
R-values:
R R work R free
0.227 0.225 0.263
Expression system: Escherichia coli BL21(DE3)

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Citations

4 citation in other articles

Catalytic Control of Spiroketal Formation in Rubromycin Polyketide Biosynthesis.
Toplak et al. (2021)
3 more