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› 7oyy

X-ray diffraction
1.36Å resolution

E.coli's putrescine receptor variant PotF/D (4JDF) with mutation S247D in complex with spermidine

Released:
DOI: 10.2210/pdb7oyy/pdb
Model geometry
Fit model/data
PDB entry 7oyy coloured by chain, front view.
PDB entry 7oyy coloured by chain, side view.
PDB entry 7oyy coloured by chain, top view.
Source organism: Escherichia coli K-12
Primary publication:
Fine-tuning spermidine binding modes in the putrescine binding protein PotF.
Kröger P, Shanmugaratnam S, Scheib U, Höcker B
OpenAccess logo J Biol Chem 297 101419 (2021)
PMID: 34801550

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-151913 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Putrescine-binding periplasmic protein PotF Chain: A
Molecule details ›
Chain: A
Length: 352 amino acids
Theoretical weight: 39.57 KDa
Source organism: Escherichia coli K-12
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P31133 (Residues: 27-370; Coverage: 100%)
Gene names: JW0838, b0854, potF
Sequence domains: Bacterial extracellular solute-binding protein

Ligands and Environments

5 bound ligands:
Ligand SPD 1 x SPD
Ligand GOL 1 x GOL
Ligand EDO 3 x EDO
Ligand PEG 1 x PEG
Ligand CL 1 x CL
No modified residues

Experiments and Validation Details

wwPDB Validation report is not available for this entry.
X-ray source: BESSY BEAMLINE 14.2
Spacegroup: P212121
Unit cell:
a: 37.059Å b: 82.146Å c: 111.071Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.157 0.156 0.186
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

2 citation in other articles

Retracing the evolution of a modern periplasmic binding protein.
Michel et al. (2023)
1 more

1 mention without citation

Fine-tuning spermidine binding modes in the putrescine binding protein PotF.
Kröger et al. (2021)