› 7p0h

X-ray diffraction
2.5Å resolution

Crystal structure of Helicobacter pylori ComF fused to an artificial alphaREP crystallization helper(named B2)

Released:
DOI: 10.2210/pdb7p0h/pdb
PDB entry 7p0h coloured by chain, front view.
PDB entry 7p0h coloured by chain, side view.
PDB entry 7p0h coloured by chain, top view.
Source organism: synthetic construct
Primary publication:
ComFC mediates transport and handling of single-stranded DNA during natural transformation.
Damke PP, Celma L, Kondekar SM, Di Guilmi AM, Marsin S, Dépagne J, Veaute X, Legrand P, Walbott H, Vercruyssen J, Guérois R, Quevillon-Cheruel S, Radicella JP
Nat Commun 13 1961 (2022)
PMID: 35414142

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-127809 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Amidophosphoribosyltransferase Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 427 amino acids
Theoretical weight: 47.12 KDa
Source organism: synthetic construct
Expression system: Escherichia coli 'BL21-Gold(DE3)pLysS AG'
UniProt:
  • Canonical: O26008 (Residues: 1-191; Coverage: 100%)
Gene name: HP_1473

Ligands and Environments

4 bound ligands:
Ligand ZN 4 x ZN
Ligand PRP 4 x PRP
Ligand MG 8 x MG
Ligand GOL 14 x GOL
1 modified residue:
Ligand MSE 8 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: SOLEIL BEAMLINE PROXIMA 2
Spacegroup: P1
Unit cell:
a: 57.88Å b: 87.98Å c: 122.79Å
α: 80.23° β: 76.44° γ: 76.39°
R-values:
R R work R free
0.222 0.221 0.245
Expression system: Escherichia coli 'BL21-Gold(DE3)pLysS AG'

Quick links

Citations

1 review citation

DNA Transport through the Dynamic Type IV Secretion System.
Ryan et al. (2023)
1 other article