› 7p4k

X-ray diffraction
2.15Å resolution

Function and Biology Details

Reactions catalysed: 
(9S,10S)-10-hydroxy-9-(phosphonooxy)octadecanoate + H(2)O = (9S,10S)-9,10-dihydroxyoctadecanoate + phosphate
An epoxide + H(2)O = a glycol
Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-152745 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Bifunctional epoxide hydrolase 2 Chains: A, B
Molecule details ›
Chains: A, B
Length: 346 amino acids
Theoretical weight: 39.65 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P34913 (Residues: 219-219, 222-555; Coverage: 60%)
Gene name: EPHX2
Sequence domains: alpha/beta hydrolase fold

Ligands and Environments

1 bound ligand:
Ligand 5IV 2 x 5IV
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SLS BEAMLINE X06SA
Spacegroup: C2
Unit cell:
a: 127.82Å b: 79.326Å c: 88.599Å
α: 90° β: 126.65° γ: 90°
R-values:
R R work R free
0.18 0.177 0.223
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

4 citation in other articles

Computing the relative binding affinity of ligands based on a pairwise binding comparison network.
Yu et al. (2023)
3 more