› 7p7s

Electron Microscopy
3Å resolution

PoxtA-EQ2 antibiotic resistance ABCF bound to E. faecalis 70S ribosome, state II

Released:
DOI: 10.2210/pdb7p7s/pdb
PDB entry 7p7s coloured by chain, front view.
PDB entry 7p7s coloured by chain, side view.
PDB entry 7p7s coloured by chain, top view.
Source organism: Enterococcus faecalis
Primary publication:
Structural basis for PoxtA-mediated resistance to phenicol and oxazolidinone antibiotics.
Crowe-McAuliffe C, Murina V, Turnbull KJ, Huch S, Kasari M, Takada H, Nersisyan L, Sundsfjord A, Hegstad K, Atkinson GC, Pelechano V, Wilson DN, Hauryliuk V
OpenAccess logo Nat Commun 13 1860 (2022)
PMID: 35387982
Related structures: EMD-13243

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero 53-mer (preferred)
PDBe Complex ID:
PDB-CPX-103283 (preferred)
Entry contents:
48 distinct polypeptide molecules
5 distinct RNA molecules
Macromolecules (53 distinct):
ARE-ABC-F family resistance factor PoxtA Chain: 0
Molecule details ›
Chain: 0
Length: 586 amino acids
Theoretical weight: 67.89 KDa
Source organism: Enterococcus faecalis
Expression system: Enterococcus faecalis
Large ribosomal subunit protein uL29 Chain: 1
Molecule details ›
Chain: 1
Length: 62 amino acids
Theoretical weight: 7.34 KDa
Source organism: Enterococcus faecalis
UniProt:
  • Canonical: A0A1B4XKV0 (Residues: 1-62; Coverage: 100%)
Gene names: DAI12_01095, DAI13_00830, EGW70_04395, EU507_10375, EY666_01555, GTI81_10935, H9Q64_09025, KUB3007_C01450, NCTC13379_00402, NCTC370_00347, P0E79_05460, rpmC
Sequence domains: Ribosomal L29 protein
Large ribosomal subunit protein uL30 Chain: 2
Large ribosomal subunit protein bL31B Chain: 3
Large ribosomal subunit protein bL32 Chain: 4
Large ribosomal subunit protein bL33 Chain: 5
Large ribosomal subunit protein bL34 Chain: 6
Large ribosomal subunit protein bL35 Chain: 7
Large ribosomal subunit protein bL36 Chain: 8
Large ribosomal subunit protein uL1 Chain: F
Large ribosomal subunit protein uL2 Chain: G
Large ribosomal subunit protein uL3 Chain: H
Large ribosomal subunit protein uL4 Chain: I
Large ribosomal subunit protein uL5 Chain: J
Large ribosomal subunit protein uL6 Chain: K
Large ribosomal subunit protein uL13 Chain: M
Large ribosomal subunit protein uL14 Chain: N
Large ribosomal subunit protein uL15 Chain: O
Large ribosomal subunit protein uL16 Chain: P
Large ribosomal subunit protein bL17 Chain: Q
Large ribosomal subunit protein uL18 Chain: R
Large ribosomal subunit protein bL19 Chain: S
Large ribosomal subunit protein bL20 Chain: T
Large ribosomal subunit protein bL21 Chain: U
Large ribosomal subunit protein uL22 Chain: V
Large ribosomal subunit protein uL23 Chain: W
Large ribosomal subunit protein uL24 Chain: X
Large ribosomal subunit protein bL27 Chain: Y
Large ribosomal subunit protein bL28 Chain: Z
Small ribosomal subunit protein uS2 Chain: c
Small ribosomal subunit protein uS3 Chain: d
Small ribosomal subunit protein uS4 Chain: e
Small ribosomal subunit protein uS5 Chain: f
Small ribosomal subunit protein bS6 Chain: g
Small ribosomal subunit protein uS7 Chain: h
Small ribosomal subunit protein uS8 Chain: i
Small ribosomal subunit protein uS9 Chain: j
Small ribosomal subunit protein uS10 Chain: k
Small ribosomal subunit protein uS11 Chain: l
Small ribosomal subunit protein uS12 Chain: m
Small ribosomal subunit protein uS13 Chain: n
Small ribosomal subunit protein uS14 Chain: o
Small ribosomal subunit protein uS15 Chain: p
Small ribosomal subunit protein bS16 Chain: q
Small ribosomal subunit protein uS17 Chain: r
Small ribosomal subunit protein bS18 Chain: s
Small ribosomal subunit protein uS19 Chain: t
Small ribosomal subunit protein bS20 Chain: u
23S ribosomal RNA Chain: A
5S ribosomal RNA Chain: B
tRNA-fMet Chain: D
16S ribosomal RNA Chain: a
Messenger RNA Chain: b

Ligands and Environments

6 bound ligands:
Ligand ATP 2 x ATP
Ligand MG 189 x MG
Ligand ZN 4 x ZN
Ligand K 152 x K
Ligand PUT 1 x PUT
Ligand SCM 1 x SCM
No modified residues

Experiments and Validation Details

wwPDB Validation report is not available for this EM entry.
Resolution: 3Å
Relevant EMDB volumes: EMD-13243
Expression system: Enterococcus faecalis

Quick links

Citations

4 review citations

Vancomycin Resistance in Enterococcus and Staphylococcus aureus.
Li et al. (2022)
3 more

2 mentions without citation

Development of a Fluorescence Polarization Assay for Multi-Determination of 10 Aminoglycosides in Pork Muscle Sample Based on Ribosomal Protein S12 and Studying Its Recognition Mechanism.
Xia et al. (2022)
1 more