7p8k

X-ray diffraction
2.65Å resolution

Crystal structure of in planta processed AvrRps4 in complex with the WRKY domain of RRS1

Released:

Function and Biology Details

Biochemical function:
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
PDBe Complex ID:
PDB-CPX-111278 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Disease resistance protein RRS1 Chain: B
Molecule details ›
Chain: B
Length: 80 amino acids
Theoretical weight: 9.29 KDa
Source organism: Arabidopsis thaliana
Expression system: Escherichia coli
UniProt:
  • Canonical: C4B7M5 (Residues: 1195-1273; Coverage: 6%)
Gene names: RCH2, RRS1, RRS1-R, WRKY52
Sequence domains: WRKY DNA -binding domain
Avirulence protein AvrRps4-like C-terminal domain-containing protein Chain: A
Molecule details ›
Chain: A
Length: 90 amino acids
Theoretical weight: 10.1 KDa
Source organism: Pseudomonas syringae
Expression system: Escherichia coli
UniProt:
  • Canonical: Q52432 (Residues: 134-220; Coverage: 40%)
Gene name: avrRps4
Sequence domains: Avirulence protein, C-terminal domain

Ligands and Environments

1 bound ligand:
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: DIAMOND BEAMLINE I04-1
Spacegroup: P6122
Unit cell:
a: 105.607Å b: 105.607Å c: 66.987Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.247 0.245 0.284
Expression system: Escherichia coli