› 7pd6

X-ray diffraction
2Å resolution

Crystal structure of Lymnaea stagnalis Acetylcholine-binding protein (Ls-AChBP) Q55R/M114V double mutant complexed with Sulfoxaflor

Released:
DOI: 10.2210/pdb7pd6/pdb
Model geometry
Fit model/data
PDB entry 7pd6 coloured by chain, front view.
PDB entry 7pd6 coloured by chain, side view.
PDB entry 7pd6 coloured by chain, top view.
Source organism: Lymnaea stagnalis
Primary publication:
Structural Biology-Guided Design, Synthesis, and Biological Evaluation of Novel Insect Nicotinic Acetylcholine Receptor Orthosteric Modulators.
Montgomery M, Rendine S, Zimmer CT, Elias J, Schaetzer J, Pitterna T, Benfatti F, Skaljac M, Bigot A
J Med Chem 65 2297-2312 (2022)
PMID: 34986308

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo pentamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-157653 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Acetylcholine-binding protein Chains: AaA, BaB, CaC, DaD, EaE, FaF, GaG, HaH, IaI, JJJ
Molecule details ›
Chains: AaA, BaB, CaC, DaD, EaE, FaF, GaG, HaH, IaI, JJJ
Length: 210 amino acids
Theoretical weight: 23.82 KDa
Source organism: Lymnaea stagnalis
Expression system: Komagataella pastoris
UniProt:
  • Canonical: P58154 (Residues: 21-229; Coverage: 100%)
Sequence domains: Neurotransmitter-gated ion-channel ligand binding domain

Ligands and Environments

1 bound ligand:
Ligand 7II 9 x 7II
No modified residues

Experiments and Validation Details

wwPDB Validation report is not available for this entry.
X-ray source: DIAMOND BEAMLINE I24
Spacegroup: P2
Unit cell:
a: 115.886Å b: 74.367Å c: 130.058Å
α: 90° β: 101.645° γ: 90°
R-values:
R R work R free
0.208 0.206 0.231
Expression system: Komagataella pastoris

Quick links

Citations

1 citations in other articles

Efficient Expression in Leishmania tarentolae (LEXSY) of the Receptor-Binding Domain of the SARS-CoV-2 S-Protein and the Acetylcholine-Binding Protein from Lymnaea stagnalis.
Son et al. (2024)