› 7pel

Electron Microscopy
3.34Å resolution

CryoEM structure of simian T-cell lymphotropic virus intasome in complex with PP2A regulatory subunit B56 gamma

Released:
DOI: 10.2210/pdb7pel/pdb
PDB entry 7pel coloured by chain, front view.
PDB entry 7pel coloured by chain, side view.
PDB entry 7pel coloured by chain, top view.
Source organisms:
Primary publication:
Cryo-EM structure of the deltaretroviral intasome in complex with the PP2A regulatory subunit B56γ.
Barski MS, Minnell JJ, Hodakova Z, Pye VE, Nans A, Cherepanov P, Maertens GN
Nat Commun 11 5043 (2020)
PMID: 33028863
Related structures: EMD-11052

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero decamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-121789 (preferred)
Entry contents:
2 distinct polypeptide molecules
2 distinct DNA molecules
Macromolecules (4 distinct):
Pol protein Chains: A, B, D, E
Molecule details ›
Chains: A, B, D, E
Length: 301 amino acids
Theoretical weight: 33.89 KDa
Source organism: Simian T-lymphotropic virus 1
Expression system: Escherichia coli
UniProt:
  • Canonical: Q4QY51 (Residues: 600-896; Coverage: 33%)
Gene name: pol
Sequence domains:
PC4 and SFRS1-interacting protein; Serine/threonine-protein phosphatase 2A 56 kDa regulatory subunit gamma isoform Chains: C, F
Molecule details ›
Chains: C, F
Length: 697 amino acids
Theoretical weight: 80.39 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: O75475 (Residues: 1-325; Coverage: 61%)
  • Canonical: Q13362 (Residues: 11-380; Coverage: 71%)
Gene names: DFS70, KIAA0044, LEDGF, PPP2R5C, PSIP1, PSIP2
Sequence domains:
DNA (5'-D(*AP*CP*TP*GP*TP*GP*TP*TP*TP*GP*GP*CP*GP*CP*TP*TP*CP*TP*CP*TP*C)-3') Chains: K, M
Molecule details ›
Chains: K, M
Length: 30 nucleotides
Theoretical weight: 9.22 KDa
DNA (5'-D(*GP*AP*GP*AP*GP*AP*AP*GP*CP*GP*CP*CP*AP*AP*AP*CP*AP*CP*A)-3') Chains: L, N
Molecule details ›
Chains: L, N
Length: 28 nucleotides
Theoretical weight: 8.59 KDa

Ligands and Environments

1 bound ligand:
Ligand ZN 4 x ZN
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 3.34Å
Relevant EMDB volumes: EMD-11052
Expression system: Escherichia coli

Quick links

Citations

6 review citations

Multimodal Functionalities of HIV-1 Integrase.
Engelman et al. (2022)
5 more