› 7pjh

X-ray diffraction
2.35Å resolution

Crystal structure of the human spliceosomal maturation factor AAR2 bound to the RNAse H domain of PRPF8

Released:
DOI: 10.2210/pdb7pjh/pdb
PDB entry 7pjh coloured by chain, front view.
PDB entry 7pjh coloured by chain, side view.
PDB entry 7pjh coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Structural and functional investigation of the human snRNP assembly factor AAR2 in complex with the RNase H-like domain of PRPF8.
Preussner M, Santos KF, Alles J, Heroven C, Heyd F, Wahl MC, Weber G
Acta Crystallogr D Struct Biol 78 1373-1383 (2022)
PMID: 36322420

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-180107 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Protein AAR2 homolog Chain: A
Molecule details ›
Chain: A
Length: 401 amino acids
Theoretical weight: 45.68 KDa
Source organism: Homo sapiens
Expression system: Trichoplusia ni
UniProt:
  • Canonical: Q9Y312 (Residues: 1-384; Coverage: 97%)
Gene names: AAR2, C20orf4, CGI-23, PRO0225
Sequence domains:
Pre-mRNA-processing-splicing factor 8 Chain: B
Molecule details ›
Chain: B
Length: 259 amino acids
Theoretical weight: 29.71 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q6P2Q9 (Residues: 1758-2016; Coverage: 11%)
Gene names: PRPC8, PRPF8
Sequence domains: PRP8 domain IV core

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: PETRA III, EMBL c/o DESY BEAMLINE P14 (MX2)
Spacegroup: C2
Unit cell:
a: 145.28Å b: 57.26Å c: 111.23Å
α: 90° β: 112.74° γ: 90°
R-values:
R R work R free
0.191 0.189 0.235
Expression systems:
  • Trichoplusia ni
  • Escherichia coli BL21(DE3)

Quick links

Citations

1 mention without citation

Structural and functional investigation of the human snRNP assembly factor AAR2 in complex with the RNase H-like domain of PRPF8.
Preussner et al. (2022)