› 7pji

X-ray diffraction
1.65Å resolution

Crystal structure of Pseudomonas aeruginosa guaB (IMP dehydrogenase) bound to ATP and GDP at 1.65A resolution

Released:
DOI: 10.2210/pdb7pji/pdb
PDB entry 7pji coloured by chain, front view.
PDB entry 7pji coloured by chain, side view.
PDB entry 7pji coloured by chain, top view.
Source organism: Pseudomonas aeruginosa
Primary publication:
Diversity of mechanisms to control bacterial GTP homeostasis by the mutually exclusive binding of adenine and guanine nucleotides to IMP dehydrogenase.
Fernández-Justel D, Marcos-Alcalde Í, Abascal F, Vidaña N, Gómez-Puertas P, Jiménez A, Revuelta JL, Buey RM
Protein Sci 31 e4314 (2022)
PMID: 35481629

Function and Biology Details

Reaction catalysed: 
Inosine 5'-phosphate + NAD(+) + H(2)O = xanthosine 5'-phosphate + NADH
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo octamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-100415 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Inosine-5'-monophosphate dehydrogenase Chains: A, B
Molecule details ›
Chains: A, B
Length: 492 amino acids
Theoretical weight: 52.05 KDa
Source organism: Pseudomonas aeruginosa
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: A0A069Q6I8 (Residues: 1-489; Coverage: 100%)
Gene names: CAZ10_17565, CSB93_2499, DT376_00630, DY940_09800, GNQ48_05465, GUL26_01290, IPC1295_13460, IPC737_18950, NCTC13621_00582, PAERUG_P19_London_7_VIM_2_05_10_06566, guaB
Sequence domains:

Ligands and Environments

6 bound ligands:
Ligand ATP 2 x ATP
Ligand GDP 2 x GDP
Ligand IMP 2 x IMP
Ligand MG 2 x MG
Ligand K 2 x K
Ligand ACT 4 x ACT
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: DIAMOND BEAMLINE I04
Spacegroup: I4
Unit cell:
a: 120.947Å b: 120.947Å c: 145.487Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.173 0.172 0.195
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

2 review citations

The gateway to guanine nucleotides: Allosteric regulation of IMP dehydrogenases.
Buey et al. (2022)
1 more

3 mentions without citation

Diversity of mechanisms to control bacterial GTP homeostasis by the mutually exclusive binding of adenine and guanine nucleotides to IMP dehydrogenase.
Fernández-Justel et al. (2022)
2 more