› 7pu0

X-ray diffraction
2.2Å resolution

Crystal Structure of Two-Domain Laccase mutant H165A/M199G from Streptomyces griseoflavus

Released:
DOI: 10.2210/pdb7pu0/pdb
PDB entry 7pu0 coloured by chain, front view.
PDB entry 7pu0 coloured by chain, side view.
PDB entry 7pu0 coloured by chain, top view.
Source organism: Streptomyces griseoflavus
Primary publication:
Engineering the Catalytic Properties of Two-Domain Laccase from Streptomyces griseoflavus Ac-993.
Kolyadenko I, Scherbakova A, Kovalev K, Gabdulkhakov A, Tishchenko S
Int J Mol Sci 23 (2021)
PMID: 35008493

Function and Biology Details

Reaction catalysed: 
4 benzenediol + O(2) = 4 benzosemiquinone + 2 H(2)O
Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-102518 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Two-domain laccase Chains: A, B, C, D, E, F
Molecule details ›
Chains: A, B, C, D, E, F
Length: 283 amino acids
Theoretical weight: 30.75 KDa
Source organism: Streptomyces griseoflavus
Expression system: Escherichia coli
UniProt:
  • Canonical: A0A0M4FJ81 (Residues: 40-322; Coverage: 88%)
Sequence domains:

Ligands and Environments

2 bound ligands:
Ligand CU 24 x CU
Ligand NA 2 x NA
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU MICROMAX-003
Spacegroup: P21
Unit cell:
a: 76.235Å b: 94.591Å c: 120.867Å
α: 90° β: 91.388° γ: 90°
R-values:
R R work R free
0.158 0.157 0.188
Expression system: Escherichia coli

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Citations

4 citation in other articles

Does Tyrosine Protect S. coelicolor Laccase from Oxidative Degradation or Act as an Extended Catalytic Site?
Kielb et al. (2022)
3 more

2 mentions without citation

Engineering the Catalytic Properties of Two-Domain Laccase from Streptomyces griseoflavus Ac-993.
Kolyadenko et al. (2021)
1 more